RNA synthesis in cells infected with herpes simplex virus. X. Properties of viral symmetric transcripts and of double-stranded RNA prepared from them
| Autor: | Bernard Roizman, B. Jacquemont |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Hot Temperature
Transcription Genetic RNase P Immunology RNA-dependent RNA polymerase Biology Nucleic Acid Denaturation Virus Replication Microbiology Cell Line chemistry.chemical_compound Transcription (biology) Virology RNA polymerase I Simplexvirus Laryngeal Neoplasms Base Sequence Nucleic Acid Hybridization RNA Nuclease protection assay Non-coding RNA Molecular biology chemistry Insect Science Carcinoma Squamous Cell RNA Viral DNA Research Article |
| Zdroj: | Journal of Virology. 15:707-713 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.15.4.707-713.1975 |
| Popis: | HEp-2 cells infected with herpes simplex 1 virus contain RNA transcripts capable of forming double-stranded (DS) RNA on annealing. The properties of purified DS RNA were as follows (i) DS RNA is resistant to depolymerization by RNase A or T-1 in 2 times 0.15 M NaCl, plus 0.015 M sodium citrate (SSC) but not 0.1 times SSC or following thermal denaturation. (ii) The Tm of the viral DS RNA was 100 C in 0.1 times SSC. (iii) Undenatured DS RNA does not hybridize with viral DNA: upon denaturation, excess unlabeled RNA drove 50 to 55% of labeled DNA into DNA-RNA hybrid. The kinetics of hybridization indicate that the DS RNA consists of at least two populations of transcripts arising from 29 and 26% of viral DNA and differing 40-fold in molar concentration. |
| Databáze: | OpenAIRE |
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