Effect of TERT on the growth of fibrosarcoma via caspase-3, survivin and PKB
| Autor: | Qiuye Ma, Xuehua Qu, Hongsuo Liang, Yidong Yu, Shan Cong, Linlin Dai |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Cancer Research Gene knockdown Oncogene Cell growth Caspase 3 Articles Biology medicine.disease Blot 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Oncology 030220 oncology & carcinogenesis Survivin medicine Cancer research Telomerase reverse transcriptase Fibrosarcoma neoplasms |
| Zdroj: | Oncology letters. 14(2) |
| ISSN: | 1792-1074 |
| Popis: | The present study explored the effect of telomerase reverse transcriptase (TERT) on the growth and apoptosis of fibrosarcoma, and investigated the potential molecular signalling pathways underlying its effect. A plasmid was constructed in order to overexpress TERT and siRNA was used to knockdown TERT. The effect of TERT on fibrosarcoma cells in vitro was studied by performing reverse transcription-quantitative PCR and western blotting to determine the expression of p53, survivin, caspase-3, caspase-7 and PKB. Knockdown of TERT suppressed cell growth, decreased fibrosarcoma volume, decreased survivin and PKB expression, and increased caspase-3 expression. The results of the present study suggest that TERT regulates the growth of fibrosarcoma in vitro and in vivo, and that this is associated with the expression of caspase-3 and survivin, in addition to the PKB signalling pathway. |
| Databáze: | OpenAIRE |
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