An AP1 binding site upstream of the kappa immunoglobulin intron enhancer binds inducible factors and contributes to expression
| Autor: | R P Podzorski, B Van Ness, J T Schanke, Adriana Marcuzzi |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Proto-Oncogene Proteins c-jun
Molecular Sequence Data Biology Immunoglobulin light chain Lymphocyte Activation DNA-binding protein Immunoglobulin kappa-Chains Mice Genetics Animals Humans Binding site Enhancer Transcription factor Regulation of gene expression B-Lymphocytes Binding Sites Base Sequence Genes Immunoglobulin Intron Gene Expression Regulation Developmental DNA Molecular biology Introns DNA-Binding Proteins Transcription Factor AP-1 AP-1 transcription factor Enhancer Elements Genetic Trans-Activators Proto-Oncogene Proteins c-fos HeLa Cells |
| Zdroj: | Nucleic acids research. 22(24) |
| ISSN: | 0305-1048 |
| Popis: | Expression of the kappa immunoglobulin light chain gene requires developmental- and tissue-specific regulation by trans-acting factors which interact with two distinct enhancer elements. A new protein-DNA interaction has been identified upstream of the intron enhancer, within the matrix-associated region of the J-C intron. The binding activity is greatly inducible in pre-B cells by bacterial lipopolysaccharide and interleukin-1 but specific complexes are found at all stages of B cell development tested. The footprinted binding site is homologous to the consensus AP1 motif. The protein components of this complex are specifically competed by an AP1 consensus motif and were shown by supershift to include c-Jun and c-Fos, suggesting that this binding site is an AP1 motif and that the Jun and Fos families of transcription factors play a role in the regulation of the kappa light chain gene. Mutation of the AP1 motif in the context of the intron enhancer was shown to decrease enhancer-mediated activation of the promoter in both pre-B cells induced with LPS and constitutive expression in mature B cells. |
| Databáze: | OpenAIRE |
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