Evolutionary recruitment of flexible Esrp-dependent splicing programs into diverse embryonic morphogenetic processes

Autor: Claudia Cuomo, Ylenia D’Agostino, Claudia Racioppi, Ana Riesgo, Rosaria Esposito, Lucia Fanlo, Yamile Marquez, Giovanna Benvenuto, Filomena Ristoratore, Salvatore D'Aniello, Enrique Navas-Perez, Jon Permanyer, Jordi Garcia-Fernàndez, Manuel Irimia, Andre Gohr, Antonio Torres-Méndez, Demian Burguera, Elisa Martí, Lionel Christiaen, Maria Ina Arnone, Beatriz Albuixech-Crespo, Antonietta Spagnuolo
Přispěvatelé: Universitat de Barcelona, European Research Council, Ministerio de Economía y Competitividad (España), Institución Catalana de Investigación y Estudios Avanzados, Generalitat de Catalunya, Universidad de Barcelona, EMBO, Garcia-Fernàndez, Jordi [0000-0001-5677-5970], Benvenuto, Giovanna [0000-0001-7155-2935], Torres-Mendéz, Antonio [0000-0002-1241-8629], Christiaen, Lionel A. [0000-0001-5930-5667], Ristoratore, Filomena [0000-0002-6023-9537], Irimia, Manuel [0000-0002-2179-2567], Garcia-Fernàndez, Jordi, Benvenuto, Giovanna, Torres-Mendéz, Antonio, Christiaen, Lionel A., Ristoratore, Filomena, Irimia, Manuel
Předmět:
0301 basic medicine
Male
Embryology
General Physics and Astronomy
Sequence Homology
Ectoderm
Esrp genes
Morphogenesis
Developmental
Molecular genetics
Animal Structures
lcsh:Science
Zebrafish
Lancelets
Multidisciplinary
Deuterostome
biology
Morfogènesi
Gene Expression Regulation
Developmental
Vertebrate
RNA-Binding Proteins
Exons
Biological Evolution
Amino Acid
medicine.anatomical_structure
Gene Knockdown Techniques
RNA splicing
Animals
CRISPR-Cas Systems
Embryonic Development
Epithelial-Mesenchymal Transition
Female
Mutation
RNA Splicing
Sequence Homology
Amino Acid
Signal Transduction
Strongylocentrotus purpuratus
Urochordata
Evolutionary developmental biology
Science
Organogenesis
Article
General Biochemistry
Genetics and Molecular Biology
Genètica molecular
03 medical and health sciences
biology.animal
Regulació genètica
medicine
Transcriptomics
Genetic regulation
Embriologia
General Chemistry
biology.organism_classification
Embryonic stem cell
030104 developmental biology
Gene Expression Regulation
Evolutionary biology
lcsh:Q
Zdroj: Recercat. Dipósit de la Recerca de Catalunya
instname
Dipòsit Digital de la UB
Universidad de Barcelona
Nature Communications, Vol 8, Iss 1, Pp 1-15 (2017)
Digital.CSIC. Repositorio Institucional del CSIC
Nature Communications
Popis: Epithelial-mesenchymal interactions are crucial for the development of numerous animal structures. Thus, unraveling how molecular tools are recruited in different lineages to control interplays between these tissues is key to understanding morphogenetic evolution. Here, we study Esrp genes, which regulate extensive splicing programs and are essential for mammalian organogenesis. We find that Esrp homologs have been independently recruited for the development of multiple structures across deuterostomes. Although Esrp is involved in a wide variety of ontogenetic processes, our results suggest ancient roles in non-neural ectoderm and regulating specific mesenchymal-to-epithelial transitions in deuterostome ancestors. However, consistent with the extensive rewiring of Esrp-dependent splicing programs between phyla, most developmental defects observed in vertebrate mutants are related to other types of morphogenetic processes. This is likely connected to the origin of an event in Fgfr, which was recruited as an Esrp target in stem chordates and subsequently co-opted into the development of many novel traits in vertebrates.
This work has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No ERC-StG-LS2-637591 to M.I.), the Spanish Ministry of Economy and Competitiveness (grant BFU2014-58908P to J.G.-F, BFU2014-55076-P to M.I., and the ‘Centro de Excelencia Severo Ochoa 2013–2017’, SEV-2012-0208), and ICREA - Generalitat de Catalunya (Academia Prize to J.G.-F). We acknowledge the support of the CERCA Programme/Generalitat de Catalunya. D.B. held an APIF fellowship from University of Barcelona, Y.M. an EMBO Long Term postdoctoral fellowship (ALTF 1505-2015), C.R. an EMBO long-term fellowship (ALTF 1608-2014), ATM an FPI-SO fellowship.
Databáze: OpenAIRE
Externí odkaz: