Lipid-load in peripheral blood mononuclear cells: Impact of food-consumption, dietary-macronutrients, extracellular lipid availability and demographic factors
| Autor: | Nousheen Zaidi, Hina Usman, Rida Rashid, Muhammad Shahjahan, Shahida Hasnain, Rimsha Munir, Fatima Ameer |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Food consumption 030204 cardiovascular system & hematology Biology Biochemistry Peripheral blood mononuclear cell 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Dietary Carbohydrates medicine Extracellular Humans Macronutrient composition Cells Cultured Triglycerides Metabolic health Triglyceride hemic and immune systems General Medicine Dietary Fats Lipids Cholesterol 030104 developmental biology Endocrinology chemistry Immunology Leukocytes Mononuclear lipids (amino acids peptides and proteins) |
| Zdroj: | Biochimie. 135:104-110 |
| ISSN: | 0300-9084 |
| DOI: | 10.1016/j.biochi.2017.01.015 |
| Popis: | Lipid-load in peripheral blood mononuclear cells (PBMCs) has recently gained attention of the researchers working on nutritional regulation of metabolic health. Previous works have indicated that the metabolic circuitries in the circulating PBMCs are influenced by dietary-intake and macronutrient composition of diet. In the present work, we analyzed the impact of diet and dietary macronutrients on PBMCs' lipid-load. The overall analyses revealed that dietary carbohydrates and fats combinatorially induce triglyceride accumulation in PBMCs. On the other hand, dietary fats were shown to induce significant decrease in PBMCs' cholesterol-load. The effects of various demographic factors -including age, gender and body-weight- on PBMCs' lipid-load were also examined. Body-weight and age were both shown to affect PBMC's lipid-load. Our study fails to provide any direct association between extracellular lipid availability and cholesterol-load in both, freshly isolated and cultured PBMCs. The presented work significantly contributes to the current understanding of the impact of food-consumption, dietary macronutrients, extracellular lipid availability and demographic factors on lipid-load in PBMCs. |
| Databáze: | OpenAIRE |
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