Characterization of zetin 1/rBSPRY, a novel binding partner of 14-3-3 proteins
| Autor: | Brigitte Anliker, Jörg Birkenfeld, Heinrich Betz, Britta Schlötcke, Dagmar Roth, Bettina Kartmann, Koji Ono |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
DNA
Complementary Transcription Genetic Tyrosine 3-Monooxygenase Molecular Sequence Data Biophysics Plasma protein binding Biology Transfection Biochemistry Cell Line Piriform cortex Two-Hybrid System Techniques medicine Animals Humans Protein Isoforms Tissue Distribution Amino Acid Sequence RNA Messenger Molecular Biology Actin In Situ Hybridization Gene Library COS cells Sequence Homology Amino Acid cDNA library Reverse Transcriptase Polymerase Chain Reaction Brain Proteins Cell Biology Blotting Northern Molecular biology Precipitin Tests Rats medicine.anatomical_structure 14-3-3 Proteins Cerebral cortex Protein Biosynthesis COS Cells Signal transduction Plasmids Protein Binding |
| Zdroj: | Biochemical and biophysical research communications. 302(3) |
| ISSN: | 0006-291X |
| Popis: | 14-3-3 proteins are ubiquitously expressed proteins which serve as central adaptors in different signal transduction cascades. In this study, yeast two-hybrid screening of a rat brain cDNA library identified a novel gene product termed zetin 1/rBSPRY that interacts with 14-3-3 zeta. The zetin 1/rBSPRY gene is ubiquitously expressed in a variety of rat tissues, with highest expression being found in testis. In adult brain, high levels of zetin 1/rBSPRY mRNA were observed in the hippocampus, cerebral cortex, and piriform cortex. Biochemical studies confirmed zetin 1/rBSPRY to interact with 14-3-3 zeta. Transient co-transfection in COS 7 cells caused a partial redistribution of zetin 1/rBSPRY into 14-3-3 zeta enriched submembranous foci at leading edges. Our results suggest a role for zetin 1/rBSPRY-14-3-3 interactions at specialized submembrane domains. |
| Databáze: | OpenAIRE |
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