Shedding light on betL*: pPL2-lux mediated real-time analysis of betL* expression in Listeria monocytogenes
| Autor: | Cormac G. M. Gahan, Colin Hill, William J. Snelling, Shauna M. Keane, Roland F. Hoffmann, Eamonn P. Culligan, Roy D. Sleator |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA Bacterial 030106 microbiology Molecular Sequence Data Bioengineering medicine.disease_cause Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Listeria monocytogenes Bacterial Proteins Transcription (biology) Stress Physiological medicine Point Mutation Amino Acid Sequence Real time analysis Promoter Regions Genetic biology Base Sequence Osmolar Concentration General Medicine Gene Expression Regulation Bacterial Sequence Analysis DNA biology.organism_classification Phenotype Molecular biology Thymine Addendum Betaine Cold Temperature 030104 developmental biology Mechanism of action chemistry Listeria medicine.symptom Carrier Proteins DNA Biotechnology |
| Popis: | Betaine uptake in Listeria monocytogenes is mediated by three independent transport systems, the simplest of which in genetic terms is the secondary transporter BetL. Using a random mutagenesis approach, based on the E. coli XL1 Red mutator strain, we identified a single point mutation in a putative promoter region upstream of the BetL coding region which leads to a significant increase in betL transcript levels under osmo- and chill-stress conditions and a concomitant increase in stress tolerance. Furthermore, the mutation appears to counter the heretofore unreported "twisted" cell morphology observed for L. monocytogenes grown at elevated osmolarities in tryptone soy broth. |
| Databáze: | OpenAIRE |
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