Hydroalcoholic extract of Tagetes erecta L. flowers, rich in the carotenoid lutein, attenuates inflammatory cytokine secretion and improves the oxidative stress in an animal model of ulcerative colitis
| Autor: | Luiz Carlos Klein-Júnior, Rita de Cássia Melo Vilhena de Andrade Fonseca da Silva, Priscila de Souza, Sérgio Faloni de Andrade, Marihá Mariott, Luisa Mota da Silva, Luísa Nathália Bolda Mariano, Bruna Longo, Ana Caroline dos Santos, Lincon Bordignon Somensi, Thaise Boeing, Tauini Caroline Santos França, Marianne Caroline Meurer, Mariéli Mees |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Lutein Antioxidant Endocrinology Diabetes and Metabolism medicine.medical_treatment Anti-Inflammatory Agents 030209 endocrinology & metabolism Flowers Pharmacology medicine.disease_cause Tagetes Superoxide dismutase Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology medicine Animals Colitis Inflammation chemistry.chemical_classification Reactive oxygen species 030109 nutrition & dietetics Nutrition and Dietetics Ethanol biology Plant Extracts Chemistry Dextran Sulfate Mucins Water medicine.disease Disease Models Animal Oxidative Stress Catalase biology.protein Cytokines Colitis Ulcerative Cytokine secretion Oxidative stress |
| Zdroj: | Nutrition Research. 66:95-106 |
| ISSN: | 0271-5317 |
| Popis: | Given the role of oxidative stress in ulcerative colitis (UC) etiology, and the amount of lutein (a carotenoid with antioxidant properties) in the dry hydroalcoholic extract of Tagetes erecta flowers (DHETE), this study investigated the intestinal anti-inflammatory properties of DHETE in an animal model of UC. The amount of lutein in the extract was determined by 1H-nuclear magnetic resonance spectroscopy, and total phenols, radical scavenger capability, cytotoxicity, and effects on reactive oxygen species and nitric oxide production were evaluated in vitro. Experimental UC was established by adding 5% dextran sulfate sodium (DSS) to drinking water, with the effects of DHETE (30-300 mg/kg, once a day for 7 days) on the morphological (colon length and weight), clinical (disease activity index and body weight loss), microscopic (histological score and mucin levels), and biochemical parameters analyzed. The lutein concentration found in DHETE was 8.2%, and DHETE scavenged 2,2-diphenyl-1-picrylhydrazyl radicals at 1000 μg/mL The exposure of intestinal epithelial cells to DHETE did not change its viability but reduced reactive oxygen species and nitric oxide production after lipopolysaccharide stimulation. In vivo, DHETE (300 mg/kg) attenuated weight loss, disease activity index, colon shortening, and histopathological changes promoted by DSS intake. Moreover, DHETE increased mucin colonic staining. The treatment with DHETE decreased myeloperoxidase activity as well as tumor necrosis factor and interleukin-6 levels. The extract also increased reduced glutathione levels and catalase activity and normalized superoxide dismutase and glutathione-S-transferase activities. In conclusion, DHETE reduced colitis severity by attenuating inflammatory cytokine secretion and improved the endogenous antioxidant defense in DSS-induced UC in mice. |
| Databáze: | OpenAIRE |
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