Diagnostic Accuracy of Electro-Chemiluminescence Immunoassay Anti-SARS-CoV-2 Serological Test

Autor: Nayab Afzal, Saba Raza, Danish Shakeel, Naila Tariq
Rok vydání: 2021
Předmět:
Zdroj: Cureus
ISSN: 2168-8184
Popis: Objective: To determine the diagnostic accuracy of fully automated electro-chemiluminescence immunoassay (ECLIA) anti-SARS-CoV-2 serological test for detection of past SARS-CoV-2 infection and to be used in seroprevalence surveys Method: A total of 426 patients who had tested for anti-SARS-CoV-2 from August 1 to 31, 2020 were selected for the study Informed consent was obtained and a questionnaire including the patient's age, gender, symptoms, and past polymerase chain reaction (PCR) status was filled by the patient Samples were analyzed for anti-SARS-CoV-2 antibodies on Roche Cobas e601 Results: The mean age of the patients was 42 43 +/- 16 67 years One hundred and five (24 6%) were PCR positive, while 321 (75 4%) were PCR negative Most patients were males 241 (56 6%) while 185(43 3%) were females Over 185(43 3%) patients presented with symptoms, and the rest of the patients 241 (56 6%) were asymptomatic Anti-SARS-CoV-2 had sensitivity 89 5%, specificity 99 06%, positive predictive value (PPV) 96 90%, negative predictive value (NPV) 96 6%, and positive likelihood ratio 4 26, while negative likelihood ratio 0 1 Diagnostic accuracy of anti-SARS-CoV-2 was 96 7% based on receiver-operating characteristic (ROC) curve analysis Conclusion: Anti-SARS-CoV-2 is very useful for the detection of past COVID-19 infection;it can be proved helpful in the identification of post-COVID complications and actual disease burden in a population
Databáze: OpenAIRE