Diagnostic Accuracy of Electro-Chemiluminescence Immunoassay Anti-SARS-CoV-2 Serological Test
Autor: | Nayab Afzal, Saba Raza, Danish Shakeel, Naila Tariq |
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Rok vydání: | 2021 |
Předmět: |
education.field_of_study
medicine.medical_specialty medicine.diagnostic_test business.industry rt-pcr Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) covid 19 Population General Engineering anti-sars-cov-2 antibodies Infectious Disease Asymptomatic Likelihood ratios in diagnostic testing Serology Allergy/Immunology Real-time polymerase chain reaction Internal medicine Immunoassay medicine Seroprevalence diagnostic accuracy Other medicine.symptom education business |
Zdroj: | Cureus |
ISSN: | 2168-8184 |
Popis: | Objective: To determine the diagnostic accuracy of fully automated electro-chemiluminescence immunoassay (ECLIA) anti-SARS-CoV-2 serological test for detection of past SARS-CoV-2 infection and to be used in seroprevalence surveys Method: A total of 426 patients who had tested for anti-SARS-CoV-2 from August 1 to 31, 2020 were selected for the study Informed consent was obtained and a questionnaire including the patient's age, gender, symptoms, and past polymerase chain reaction (PCR) status was filled by the patient Samples were analyzed for anti-SARS-CoV-2 antibodies on Roche Cobas e601 Results: The mean age of the patients was 42 43 +/- 16 67 years One hundred and five (24 6%) were PCR positive, while 321 (75 4%) were PCR negative Most patients were males 241 (56 6%) while 185(43 3%) were females Over 185(43 3%) patients presented with symptoms, and the rest of the patients 241 (56 6%) were asymptomatic Anti-SARS-CoV-2 had sensitivity 89 5%, specificity 99 06%, positive predictive value (PPV) 96 90%, negative predictive value (NPV) 96 6%, and positive likelihood ratio 4 26, while negative likelihood ratio 0 1 Diagnostic accuracy of anti-SARS-CoV-2 was 96 7% based on receiver-operating characteristic (ROC) curve analysis Conclusion: Anti-SARS-CoV-2 is very useful for the detection of past COVID-19 infection;it can be proved helpful in the identification of post-COVID complications and actual disease burden in a population |
Databáze: | OpenAIRE |
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