| Popis: |
The structural elements of the dorsal root ganglion which situated on the way of dorsal root of peripherial nerve from both side of spinal cord at intervertebral foramen level, has been studied for years and is still being studied. It is no coincidence that the morphofunctional changes occurring in the spinal dorsal root ganglion are always brought to the fore in the pathogenesis of various types of neuropathies accompanied by sensory disturbances. Aim of the research was to study deviation on the composition and ultrastructure of the connective tissue elements of spinal dorsal root ganglia used as a model for E.Coli induced inflammation. The object of the study was dorsal root ganglia taken from 20 white rats. Acute experimental endotoxemia was induced by intravenous administration of nonpurified LPS from E. coli (Serotype 0111:B4 InvivoGen, San Diego, CA 92121) at a dose of 1,0 mg/kg dissolved in saline. Intravenous administration of E.coli endotoxin leads to accumulation of large amount edematous fluid in different areas of object (pericapsular, within capsule, under capsular, between nerve elements of organ), that clearly visible under light microscope. Mechanical force of fluid caused destructive changes in capsular elements of dorsal root ganglion. These changes included the inability to identify the boundaries and the basal membrane of perineurial cells, also the chaotic irregular orientation of collagen fibers, which constitue connective tissue elements spinal dorsal root ganglia. |
