IL-1β Inhibits Human Osteoblast Migration
| Autor: | Joerg Fiedler, Anita Ignatius, Rolf E. Brenner, Nina-Emily Hengartner |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty medicine.medical_treatment Interleukin-1beta Becaplermin Bone healing Bone resorption Bone remodeling Cell Movement Internal medicine Genetics medicine Cell Adhesion Humans Insulin-Like Growth Factor I Molecular Biology Genetics (clinical) Cells Cultured Receptors Interleukin-1 Type I Osteoblasts biology Growth factor Mesenchymal stem cell Osteoblast Mesenchymal Stem Cells Articles Proto-Oncogene Proteins c-sis Recombinant Proteins Cell biology Vascular endothelial growth factor A medicine.anatomical_structure Endocrinology biology.protein Molecular Medicine Mitogen-Activated Protein Kinases Platelet-derived growth factor receptor |
| Popis: | Bone has a high capacity for self-renewal and repair. Prolonged local secretion of interleukin 1β (IL-1β), however, is known to be associated with severe bone loss and delayed fracture healing. Since induction of bone resorption by IL-1β may not sufficiently explain these pathologic processes, we investigated, in vitro, if and how IL-1β affects migration of multipotent mesenchymal stromal cells (MSC) or osteoblasts. We found that homogenous exposure to IL-1 β significantly diminished both nondirectional migration and site-directed migration toward the chemotactic factors platelet-derived growth factor (PDGF)-BB and insulinlike growth factor 1 (IGF-1) in osteoblasts. Exposure to a concentration gradient of IL-1β induced an even stronger inhibition of migration and completely abolished the migratory response of osteoblasts toward PDGF-BB, IGF-1, vascular endothelial growth factor A (VEGF-A) and the complement factor C5a. IL-1β induced extracellular signal-regulated kinases 1 and 2 (ERK1/2) and c-Jun N-terminal kinases (JNK) activation and inhibition of these signaling pathways suggested an involvement in the IL-1β effects on osteoblast migration. In contrast, basal migration of MSC and their migratory activity toward PDGF-BB was found to be unaffected by IL-1β. These results indicate that the presence of IL-1β leads to impaired recruitment of osteoblasts which might influence early stages of fracture healing and could have pathological relevance for bone remodeling in inflammatory bone disease. |
| Databáze: | OpenAIRE |
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