Stroke-Induced Brain Parenchymal Injury Drives Blood–Brain Barrier Early Leakage Kinetics: A Combined in Vivo / in Vitro Study
| Autor: | Mélanie Kuntz, Rustem Uzbekov, Vincent Berezowski, Olivier Pétrault, Régis Bordet, Laurence Fenart, Roméo Cecchelli, Maud Pétrault, Caroline Mysiorek |
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| Přispěvatelé: | Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique (LBHE), Université d'Artois (UA) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Pathology medicine.medical_specialty magnetic resonance imaging (MRI) Endothelium [SDV]Life Sciences [q-bio] Vascular permeability Biology Blood–brain barrier Lesion Brain ischemia Capillary Permeability 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine In vivo medicine Animals Cells Cultured 030304 developmental biology Evans Blue 0303 health sciences cell culture Cell Death Endothelial Cells medicine.disease Magnetic Resonance Imaging Coculture Techniques brain ischemia reperfusion Mice Inbred C57BL Oxygen Stroke Disease Models Animal Kinetics medicine.anatomical_structure Glucose Neurology chemistry Blood-Brain Barrier Neuroglia Original Article Neurology (clinical) medicine.symptom Cardiology and Cardiovascular Medicine 030217 neurology & neurosurgery |
| Zdroj: | Journal of Cerebral Blood Flow and Metabolism Journal of Cerebral Blood Flow and Metabolism, Nature Publishing Group, 2014, 34 (1), pp.95-107. ⟨10.1038/jcbfm.2013.169⟩ Journal of Cerebral Blood Flow & Metabolism; Vol 34 |
| ISSN: | 0271-678X 1559-7016 |
| DOI: | 10.1038/jcbfm.2013.169⟩ |
| Popis: | International audience; The disappointing clinical outcomes of neuroprotectants challenge the relevance of preclinical stroke models and data in defining early cerebrovascular events as potential therapeutic targets. The kinetics of blood–brain barrier (BBB) leakage after reperfusion and the link with parenchymal lesion remain debated. By using in vivo and in vitro approaches, we conducted a kinetic analysis of BBB dysfunction during early reperfusion. After 60 minutes of middle cerebral artery occlusion followed by reperfusion times up to 24 hours in mice, a non-invasive magnetic resonance imaging method, through an original sequence of diffusion-weighted imaging, determined brain water mobility in microvascular compartments (D*) apart from parenchymal compartments (apparent diffusion coefficient). An increase in D* found at 4 hours post reperfusion concurred with the onset of both Evans blue/Dextran extravasations and in vitro BBB opening under oxygen-glucose deprivation and reoxygenation (R). The BBB leakage coincided with an emerging cell death in brain tissue as well as in activated glial cells in vitro. The co-culture of BBB endothelial and glial cells evidenced a recovery of endothelium tightness when glial cells were absent or non-injured during R. Preserving the ischemic brain parenchymal cells within 4 hours of reperfusion may improve therapeutic strategies for cerebrovascular protection against stroke. |
| Databáze: | OpenAIRE |
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