Mapping the promoter for subgenomic RNA synthesis on beet necrotic yellow vein virus RNA 3
| Autor: | Kenneth Richards, Gérard Jonard, Hubert Guilley, David Gilmer, E. Balmori |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Base Sequence
Transcription Genetic Molecular Sequence Data Intron RNA-dependent RNA polymerase RNA General Medicine Biology Non-coding RNA Biochemistry Molecular biology Plant Viruses RNA editing Transcription (biology) medicine RNA Viruses RNA Viral T7 RNA polymerase Promoter Regions Genetic Small nuclear RNA Sequence Deletion medicine.drug |
| Zdroj: | Biochimie. 75:517-521 |
| ISSN: | 0300-9084 |
| DOI: | 10.1016/0300-9084(93)90056-x |
| Popis: | During infection of Tetragonia expansa leaves, RNA 3 of the quadripartite genome of beet necrotic yellow vein virus directs synthesis of a subgenomic RNA (RNA 3sub) which corresponds to the 3′-terminal 600 residues of the RNA 3 molecule. Biologically active run-off transcripts have been prepared from full-length cDNA of RNA 3 cloned behind a bacteriophage T7 RNA polymerase promoter. RNA 3 transcripts carrying deletions in the vicinity of the RNA 3sub initiation site were produced by site-directed mutagenesis at the cDNA level and then tested for their capacity to direct RNA 3sub synthesis in infected leaves. The cis-acting domain essential for normal levels of RNA 3sub production in planta (the ‘core’ promoter) did not extend in the 5′-direction beyond position −16 relative to the RNA 3sub transcription initiation site. The 3′-boundary of the core promoter domain was located somewhere between positions +100 and +208. Displacement of the promoter domain to an upstream site in RNA 3 produced a new subgenomic RNA starting at or near the predicted upstream site. |
| Databáze: | OpenAIRE |
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