Electrospun vancomycin-loaded nanofibers for management of methicillin-resistant Staphylococcus aureus-induced skin infections
| Autor: | Mahmoud Elsabahy, Mahmoud El Badry, Mohamed A El-Mokhtar, Gihan Fetih, Ayman A. Abdelaziz, Ayat Abdelkader, Mahmoud S. AbdelKarim, Ayat A. Allam, Heba A. Fathi |
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| Rok vydání: | 2020 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Drug Biocompatibility media_common.quotation_subject Nanofibers Pharmaceutical Science Microbial Sensitivity Tests 02 engineering and technology Skin infection medicine.disease_cause 030226 pharmacology & pharmacy Microbiology 03 medical and health sciences 0302 clinical medicine Vancomycin medicine Animals media_common Chemistry Staphylococcal Infections 021001 nanoscience & nanotechnology Antimicrobial medicine.disease Methicillin-resistant Staphylococcus aureus Anti-Bacterial Agents Drug Liberation Staphylococcus aureus Nanofiber 0210 nano-technology medicine.drug |
| Zdroj: | International Journal of Pharmaceutics. 586:119620 |
| ISSN: | 0378-5173 |
| DOI: | 10.1016/j.ijpharm.2020.119620 |
| Popis: | Skin damage exposes the underlying layers to bacterial invasion, leading to skin and soft tissue infections. Several pathogens have developed resistance against conventional topical antimicrobial treatments and rendered them less effective. Recently, several nanomedical strategies have emerged as a potential approach to improve therapeutic outcomes of treating bacterial skin infections. In the current study, nanofibers were utilized for topical delivery of the antimicrobial drug vancomycin and evaluated as a promising tool for treatment of topical skin infections. Vancomycin-loaded nanofibers were prepared via electrospinning technique, and vancomycin-loaded nanofibers of the optimal composition exhibited nanosized uniform smooth fibers (ca. 200 nm diameter), high drug entrapment efficiency and sustained drug release patterns over 48 h. In vitro cytotoxicity assays, using several cell lines, revealed the biocompatibility of the drug-loaded nanofibers. In vitro antibacterial studies showed sustained antibacterial activity of the vancomycin-loaded nanofibers against methicillin-resistant Staphylococcus aureus (MRSA), in comparison to the free drug. The nanofibers were then tested in animal model of superficial MRSA skin infection and demonstrated a superior antibacterial efficiency, as compared to animals treated with the free vancomycin solution. Hence, nanofibers might provide an efficient nanodevice to overcome MRSA-induced skin infections and a promising topical delivery vehicle for antimicrobial drugs. |
| Databáze: | OpenAIRE |
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