Olopatadine enhances recovery of alkali-induced corneal injury in rats

Autor: Mohamed Balaha, Samah Kandeel
Rok vydání: 2018
Předmět:
Male
Vascular Endothelial Growth Factor A
0301 basic medicine
medicine.medical_specialty
genetic structures
VEGF receptors
medicine.medical_treatment
Interleukin-1beta
Anti-Inflammatory Agents
Alkalies
General Biochemistry
Genetics and Molecular Biology
Cornea
Neovascularization
03 medical and health sciences
0302 clinical medicine
Corneal Injury
Ophthalmology
Burns
Chemical
Hypersensitivity
medicine
Animals
Rats
Wistar
General Pharmacology
Toxicology and Pharmaceutics
Olopatadine Hydrochloride
Saline
Wound Healing
biology
Caspase 3
business.industry
Anti-Inflammatory Agents
Non-Steroidal
Corneal opacity
Therapeutic effect
NF-kappa B
General Medicine
Olopatadine
Immunohistochemistry
eye diseases
Rats
Disease Models
Animal
030104 developmental biology
medicine.anatomical_structure
030221 ophthalmology & optometry
biology.protein
sense organs
medicine.symptom
business
Corneal Injuries
medicine.drug
Zdroj: Life Sciences. 207:499-507
ISSN: 0024-3205
Popis: Aims The alkali-induced corneal injury is an ocular emergency that required an immediate and effective management to preserve the normal corneal functions and transparency. Olopatadine is a fast, topically-effective anti-allergic drug, which exhibited potent anti-inflammatory and anti-angiogenic abilities in different allergic animals' models. Therefore, this study aimed to evaluate the therapeutic effect of olopatadine on alkali-induced corneal injury in rats. Materials and methods Corneal alkali injury (CI) induced in the right eyes of an eight-week-old male Wister rats, by application of 3 mm diameter filter-papers, soaked for 10 s in 1 N-NaOH, to the right eyes' corneal centers for 30 s, afterward, the filter paper removed, and the rat right eye rinsed with 20 ml normal saline. For treatment of CI, either 0.2% or 0.77% olopatadine applied topically daily for 14 days, starting immediately after the induction of CI. Key findings Olopatadine, in the present work, effectively and dose-dependently enhanced the corneal healing after alkali application, with significant reduction of the corneal opacity and neovascularization scores, besides, it suppressed the augmented corneal IL-1β, VEGF, caspase-3 levels, and nuclear NF-κB immunohistochemical expression, meanwhile it abrogated the corneal histopathological changes, induced by alkali application. Significance Olopatadine appears to be a potential treatment option for alkali-induced corneal injury.
Databáze: OpenAIRE
Externí odkaz: