LIN-39 and the EGFR/RAS/MAPK pathway regulate C. elegans vulval morphogenesis via the VAB-23 zinc finger protein
| Autor: | Erika Fröhli, Hubert Rehrauer, Sarfarazhussain Farooqui, Fritz Müller, Robin B. Gasser, Alex Hajnal, Stéphanie Kaeser-Pebernard, Mark W. Pellegrino |
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| Přispěvatelé: | University of Zurich |
| Rok vydání: | 2011 |
| Předmět: |
Cellular differentiation
Semaphorins 1309 Developmental Biology Cell Fusion 0302 clinical medicine Genes Reporter Morphogenesis Hox gene ras Zinc finger 0303 health sciences integumentary system Intracellular Signaling Peptides and Proteins Gene Expression Regulation Developmental Zinc Fingers 10124 Institute of Molecular Life Sciences Cell biology ErbB Receptors Vulval cell fate specification embryonic structures C. elegans RNA Interference Signal transduction Mitogen-Activated Protein Kinases Signal Transduction animal structures Recombinant Fusion Proteins Molecular Sequence Data 610 Medicine & health 10071 Functional Genomics Center Zurich Biology Cell fate determination hox Vulva 03 medical and health sciences Semaphorin 1312 Molecular Biology Animals Cell Lineage Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology 030304 developmental biology Homeodomain Proteins Base Sequence 570 Life sciences biology Carrier Proteins Sequence Alignment 030217 neurology & neurosurgery Biomarkers Developmental Biology Transcription Factors |
| Zdroj: | Development (Cambridge England) Development |
| ISSN: | 1477-9129 |
| Popis: | Morphogenesis represents a phase of development during which cell fates are executed. The conserved hox genes are key cell fate determinants during metazoan development, but their role in controlling organ morphogenesis is less understood. Here, we show that the C. elegans hox gene lin-39 regulates epidermal morphogenesis via its novel target, the essential zinc finger protein VAB-23. During the development of the vulva, the egg-laying organ of the hermaphrodite, the EGFR/RAS/MAPK signaling pathway activates, together with LIN-39 HOX, the expression of VAB-23 in the primary cell lineage to control the formation of the seven vulval toroids. VAB-23 regulates the formation of homotypic contacts between contralateral pairs of cells with the same sub-fates at the vulval midline by inducing smp-1 (semaphorin) transcription. In addition, VAB-23 prevents ectopic vulval cell fusions by negatively regulating expression of the fusogen eff-1. Thus, LIN-39 and the EGFR/RAS/MAPK signaling pathway, which specify cell fates earlier during vulval induction, continue to act during the subsequent phase of cell fate execution by regulating various aspects of epidermal morphogenesis. Vulval cell fate specification and execution are, therefore, tightly coupled processes. |
| Databáze: | OpenAIRE |
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