miRNA-1236 inhibits HIV-1 infection of monocytes by repressing translation of cellular factor VprBP
| Autor: | Chan-Juan Shen, Éric A. Cohen, Sidong Xiong, Li Ma, Jian-Hua Wang |
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| Rok vydání: | 2014 |
| Předmět: |
Small interfering RNA
Cellular differentiation Ubiquitin-Protein Ligases Genetic Vectors lcsh:Medicine Biology Protein Serine-Threonine Kinases Transfection Microbiology Host Specificity Monocytes Genes Reporter Virology microRNA Protein biosynthesis Humans RNA Small Interfering lcsh:Science 3' Untranslated Regions Cells Cultured Regulation of gene expression Multidisciplinary Three prime untranslated region lcsh:R virus diseases Biology and Life Sciences Translation (biology) Cell Differentiation Dendritic Cells Viral Replication Cell biology MicroRNAs HEK293 Cells Viral replication Gene Expression Regulation Gene Knockdown Techniques Protein Biosynthesis Host-Pathogen Interactions HIV-1 lcsh:Q Carrier Proteins Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 6, p e99535 (2014) |
| ISSN: | 1932-6203 |
| Popis: | Primary monocytes are refractory to HIV-1 infection and become permissive upon differentiation into monocyte-derived dendritic cells (MDDCs) or macrophages. Multiple mechanisms have been proposed to interpret HIV-1 restriction in monocytes. Human cellular miRNAs can modulate HIV-1 infection by targeting either conserved regions of the HIV-1 genome or host gene transcripts. We have recently reported that the translation of host protein pur-alpha is repressed by abundant cellular miRNAs to inhibit HIV-1 infection in monocytes. Here, we report that the transcript of another cellular factor, VprBP [Vpr (HIV-1)-binding protein], was repressed by cellular miRNA-1236, which contributes to HIV-1 restriction in monocytes. Transfection of miR-1236 inhibitors enhanced translation of VprBP in monocytes and significantly promoted viral infection; exogenous input of synthesized miR-1236 mimics into MDDCs suppressed translation of VprBP, and, accordingly, significantly impaired viral infection. Our data emphasize the role of miRNA in modulating differentiation-dependent susceptibility of the host cell to HIV-1 infection. Understanding the modulation of HIV-1 infection by cellular miRNAs may provide key small RNAs or the identification of new important protein targets regulated by miRNAs for the development of antiviral strategies. |
| Databáze: | OpenAIRE |
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