Amyloid β peptide affects erythrocyte morphology: Role of intracellular signaling pathways
| Autor: | Francesco Misiti, Simone Dinarelli, Marco Girasole |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Cell signaling
Physiology Cell Caspase 3 030204 cardiovascular system & hematology Microscopy Atomic Force Nitric Oxide RBC 030218 nuclear medicine & medical imaging Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Physiology (medical) caspase 3 medicine Humans PKC Amyloid beta peptide atomic force microscopy membrane roughness nitric oxide synthase Amyloid beta-Peptides Erythrocyte Count Signal Transduction Protein kinase C Microscopy biology Atomic Force Hematology Cell biology Nitric oxide synthase Metabolic pathway medicine.anatomical_structure chemistry biology.protein Signal transduction Cardiology and Cardiovascular Medicine |
| Popis: | BACKGROUND Circulating red blood cells (RBCs) undergo aging, a fundamental physiological phenomenon that regulates their turnover. OBJECTIVE Understanding the role of Aβ in the cross talk between cell signalling pathways and modulation of the cell structural and biomechanical properties occurring in RBCs during aging. METHODS The morphological pattern has been monitored using Atomic Force Microscopy (AFM) imaging and measuring the RBCs' plasma membrane roughness employed as a morphological parameter capable to provide information on the structure and integrity of the membrane-skeleton. RESULTS We show that treatment with Aβ accelerates the occurrence of morphological and biochemical aging markers in human RBC and influences the cell metabolism. Biochemical data demonstrate that contemporaneously to morphological alterations, Aβ triggers: (i) metabolic alterations and (ii) a complex signaling pathway involving caspase 3, protein kinase C and nitric oxide derived metabolites. CONCLUSIONS our study provides a comprehensive picture in which Aβ treatment of RBC induces changes in specific cell signalling events and/or metabolic pathways, in turns affecting the membrane-cytoskeleton interaction and the membrane integrity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|