Simultaneous development of six LC-MS-MS methods for the determination of multiple analytes in human plasma
| Autor: | Xiangyu Jiang, Timothy D J Halls, Weng Naidong, Hai-Zhi Bu, Wilson Z. Shou, Yu Luan Chen |
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| Rok vydání: | 2002 |
| Předmět: |
Analyte
Clinical Biochemistry Analytical chemistry Pharmaceutical Science Tandem mass spectrometry Clonidine Mass Spectrometry Analytical Chemistry Liquid–liquid extraction Drug Discovery Protein precipitation Humans Technology Pharmaceutical Sample preparation Albuterol Solid phase extraction Spectroscopy Chromatography Ritonavir Chemistry Extraction (chemistry) Loratadine Naltrexone Fentanyl Pharmaceutical Preparations Quantitative analysis (chemistry) Chromatography Liquid |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 28(6) |
| ISSN: | 0731-7085 |
| Popis: | Traditional sequential single analyte method development is both time-consuming and labor-intensive. In this report, a concept of simultaneously developing multiple liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS) methods were proposed. Mass spectrometric and chromatographic conditions as well as sample preparation methods for all analytes were optimized concurrently. Mass spectrometric conditions for six analytes, i.e. clonidine (CLO), albuterol (ALB), fentanyl (FEN), ritonavir (RIT), naltrexone (NAL), and loratadine (LOR), were established simultaneously using the Sciex Analyst software. LC-MS-MS sensitivities obtained using gradient elution methods on reversed-phase Inertsil ODS3 and normal phase Betasil silica columns were compared. Sample extraction methods using protein precipitation, liquid/liquid extraction, or solid-phase extraction (SPE) were evaluated. Recovery of analytes was determined. Matrix effects and interference due to endogenous compounds were investigated. Selection of a potential internal standard was discussed. |
| Databáze: | OpenAIRE |
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