Simultaneous determination of chloroquine, amodiaquine and their metabolites in human plasma, red blood cells, whole blood and urine by column liquid chromatography
| Autor: | M.C. Blayo, Eric Pussard, F. Verdier |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Chromatography
Erythrocytes Chemistry Metabolite Aqueous two-phase system Amodiaquine Chloroquine General Chemistry Urine Absorbance chemistry.chemical_compound Plasma Pharmacokinetics medicine Blood plasma fractionation Humans Spectrophotometry Ultraviolet medicine.drug Whole blood Chromatography Liquid |
| Zdroj: | Journal of chromatography. 374(1) |
| Popis: | A column liquid chromatographic method for the simultaneous determination of chloroquine, amodiaquine and their monodesethyl metabolite in human plasma, red blood cells, whole blood and urine is described. The drugs and internal standard were extracted as bases with methylene dichloride and then re-extracted into an acid aqueous phase. Separation was obtained using a reversed-phase column and a mobile phase of phosphate buffer (pH 3.0)—acetonitrile (88:12). The absorbance of the drugs was monitored at 340 nm with a sensitivity limit of 10 pmol/ml. No endogenous compound interfered at this wavelength. The mean overall recovery from each biological fluid was > 75%. This method can be applied to therapeutic, pharmacokinetic and epidemiological studies. The metabolism of these two amino-4-quinolines in humans is compared. |
| Databáze: | OpenAIRE |
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