Production of Recombinant Vaccine Cb Peritrophin-42 of Screwworm Fly in Escherichia coli and Saccharomyces cerevisiae

Autor:	April H Wardhana, Joop Van Den Heuvel, Dessy Natalia, Elizabeth Maria, S Muharsini, Savira Eka Wardani, Fifi Fitriah. Masduki
Rok vydání:	2007
Předmět:	Signal peptide biology Oligonucleotide Chemistry fungi Saccharomyces cerevisiae General Engineering medicine.disease_cause biology.organism_classification Microbiology Chrysomya bezziana QR1-502 law.invention Membrane protein law vaccine medicine Recombinant DNA Gene Escherichia coli
Zdroj:	Microbiology Indonesia, Vol 1, Iss 3 (2010)
ISSN:	2087-8575 1978-3477
DOI:	10.5454/mi.1.3.2
Popis:	The screwworm fly (Chrysomya bezziana) larva is an obligate parasite which causes myasis in mammals. Vaccination is thought to be a protective and an enviromentally friendly method for combating the pest. A gene encoding a peritrophic membrane protein Cb peritrophin-42 of C. bezziana was cloned and expressed in Escherichia coli and Saccharomyces cerevisiae.Cb peritrophin-42 fused with the outer membrane protein A signal sequence was produced as an aggregate in E. coli. Expression of an Cb peritrophin-42 gene fused with oligonucleotide of the invertase signal sequence in S. cerevisiae allowed the production of 14.4 mg l-1 soluble extracellular Cb peritrophin-42. Sheep vaccinated with recombinant Cb peritrophin-42 showed a strong immunological reaction. In vivo assay following vaccination with the recombinant Cb peritrophin-42 showed a 27% reduction in the weight of recovered larvae.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::125400f29e2f9e6aba85f4216193f3c4 https://doi.org/10.5454/mi.1.3.2 Zobrazit plný text záznamu