Peripheral blood monocytes show increased osteoclast differentiation potential compared to bone marrow monocytes
Autor: | Christina Patlaka, Juha Tuukkanen, Elina Kylmäoja, Petri Lehenkari, Göran Andersson, Sanna Turunen, Miho Nakamura |
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Rok vydání: | 2018 |
Předmět: |
musculoskeletal diseases
0301 basic medicine Cell biology Multidisciplinary biology Chemistry Stem cell research Transforming growth factor beta Peripheral blood mononuclear cell Bone resorption 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure RANKL Osteoclast biology.protein medicine lcsh:H1-99 Bone marrow lcsh:Social sciences (General) Calcitonin receptor lcsh:Science (General) lcsh:Q1-390 Tartrate-resistant acid phosphatase |
Zdroj: | Heliyon, Vol 4, Iss 9, Pp e00780-(2018) |
ISSN: | 2405-8440 |
Popis: | Bone marrow (BM) and peripheral blood (PB) derived mononuclear cells are precursors of in vitro osteoclast differentiation. However, few studies have compared the phenotypic and functional properties of osteoclasts generated from these sources and the effects of different growth factors on osteoclastogenesis. Both cell types differentiated into functional osteoclasts, but culturing the cells with or without transforming growth factor beta (TGF-β) and dexamethasone revealed differences in their osteoclastogenic capacity. When receptor activator for nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were used for differentiation, we did not observe differences in bone resorption activity or expression of osteoclastogenic genes calcitonin receptor (CR) and nuclear factor of activated T-cells (NFATc1) between the osteoclasts formed from the two sources. Addition of TGF-β and dexamethasone led to higher number of nuclei in multinuclear cells and increased expression of tartrate resistant acid phosphatase (TRACP) 5a and 5b, CR and NFATc1 in PB- derived osteoclasts depicting the higher osteoclastogenic potential and responsiveness to TGF-β and dexamethasone in PB monocytes. These results conclude that the choice of the osteoclast precursor source as well as the choice of osteoclastogenic growth factors are essential matters in determining the phenotypic characteristics of heterogeneous osteoclast populations. |
Databáze: | OpenAIRE |
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