A surface spectroscopy study of a Pseudomonas fluorescens biofilm in the presence of an immobilized air bubble
| Autor: | Mohammad Pousti, T. Lefèvre, M. Abbaszadeh Amirdehi, Jesse Greener |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Bubble
Air Microbiology Analytical chemistry Pseudomonas fluorescens 02 engineering and technology 010402 general chemistry 01 natural sciences Analytical Chemistry Phase (matter) Spectroscopy Fourier Transform Infrared Spectroscopy Instrumentation Microchannel biology Chemistry Biofilm Equipment Design Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology biology.organism_classification Atomic and Molecular Physics and Optics 0104 chemical sciences Biofilms Attenuated total reflection Absorption (chemistry) 0210 nano-technology |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 222:117163 |
| ISSN: | 1386-1425 |
| DOI: | 10.1016/j.saa.2019.117163 |
| Popis: | A linear spectral mapping technique was applied to monitor the growth of biomolecular absorption bands at the bio-interface of a nascent Pseudomonas fluorescens biofilm during and after interaction with a surface-adhered air bubble. Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectra were obtained in different locations in a microchannel with adequate spatial and temporal resolution to study the effect of a static bubble on the evolution of protein and lipid signals at the ATR crystal surface. The results reveal that the presence of a bubble during the lag phase modified levels of extracellular lipids and affected a surface restructuring process, many hours after the bubble's disappearance. |
| Databáze: | OpenAIRE |
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