Low temperature preservation of porcine semen: influence of short antimicrobial lipopeptides on sperm quality and bacterial load
| Autor: | B. Hensel, Markus Jung, Kathi Scheinpflug, K. Greber, J. Schäfer, U. Jakop, F. Schröter, Martin Schulze, Kristin Mühldorfer |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Cell biology Swine ved/biology.organism_classification_rank.species lcsh:Medicine Biology Bacterial growth medicine.disease_cause Article Microbiology 03 medical and health sciences Lipopeptides 0302 clinical medicine Semen Trueperella pyogenes medicine Animals lcsh:Science Insemination Artificial Animal biotechnology Multidisciplinary Alcaligenes faecalis Streptococcus porcinus Bacteria ved/biology Pseudomonas aeruginosa Antimicrobials Providencia stuartii lcsh:R Temperature Drug screening Antimicrobial biology.organism_classification Anti-Bacterial Agents 030104 developmental biology lcsh:Q lipids (amino acids peptides and proteins) 030217 neurology & neurosurgery Semen Preservation |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) Scientific reports, 10:13225 |
| ISSN: | 2045-2322 |
| Popis: | Antimicrobial resistance is a steadily increasing problem and poses a serious threat to global public health. Therefore, it is highly necessary to advance the development of novel antimicrobial compounds and semen preservation strategies. The aim of this study was to evaluate a low temperature, antibiotic-free preservation procedure using Androstar Premium (ASP) extender (Minitüb) with antimicrobial lipopeptides. Firstly, seven lipopeptides in two concentrations (1 × minimum inhibitory concentration (MIC)/2 × MIC) were tested on their sperm-compatibility at 17 °C. Two lipopeptides, C16-KKK-NH2 and C16-KKKK-NH2, did not negatively affect sperm quality and were further evaluated for their efficiency of bacterial growth inhibition at 5 °C. Besides an overall diminution of colony forming units, both peptides showed a reduction of bacterial subcultures (n = 103) with a decrement in Gram-positive rods from 65 (ASP w/o supplements) to 39/52 (ASP w/ C16-KKK-NH2/C16-KKKK-NH2), in Gram-positive cocci from 21 to 9/10 and in Gram-negative species from 17 to 8/5 total subcultures. Furthermore, lipopeptides revealed activity towards selected bacteria of potential concern in artificial insemination like Trueperella pyogenes, Alcaligenes faecalis, Pseudomonas aeruginosa (not C16-KKK-NH2), Pasteurella sp., Providencia stuartii, Escherichia coli (not C16-KKKK-NH2) and Streptococcus porcinus (not C16-KKKK-NH2). Consequently, both tested lipopeptides are promising candidates for alternative antibiotic-free preservation techniques of boar semen. |
| Databáze: | OpenAIRE |
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