Profiling cytokine levels in chlorhexidine and EGCG-treated odontoblast-like cells
| Autor: | Anuradha Prakki, M.R. Carrilho, Alexander Terry Stavroullakis, Céline M. Lévesque |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science Serial dilution medicine.medical_treatment Cell Catechin Cell Line Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Bicinchoninic acid assay General Materials Science MTT assay General Dentistry Odontoblasts Chlorhexidine 030206 dentistry Molecular biology 030104 developmental biology Odontoblast Cytokine medicine.anatomical_structure chemistry Mechanics of Materials Cell culture Cytokines |
| Zdroj: | Dental Materials. 34:e107-e114 |
| ISSN: | 0109-5641 |
| Popis: | To screen the effect of two compounds, chlorhexidine diacetate (CHX) and epigallocatechin-gallate (EGCG), on the levels of cytokines produced by odontoblast-like cells (MDPC-23).Cells were seeded at 24h and 48h with serial dilution of the compounds to determine cell metabolic activity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (n=3). Cells with no compound treatment were used as control (Ctr). For the highest equal non-cytotoxic compound dilution tested at 48h cell treatment, total protein concentration was measured using a Pierce bicinchoninic acid (BCA) assay (n=3), and expression of 23 cytokines was analyzed using the Bio-Plex cytokine assay (n=2). Data were analyzed by one-way ANOVA and Tukey's test (α=5%).The MTT assay revealed that at 24h and 48h, CHX and EGCG did not reduce cell metabolic activity at concentrations of 2.5-20μM (CHX) and 2.5-160μM (EGCG), respectively (p0.05). At 48h, total protein levels were consistent across all groups for 20μM compound dilution (Ctr: 1.04mg/mL; CHX: 0.98mg/mL; and EGCG: 1.06mg/mL). At 20μM dilution, both CHX and EGCG significantly increased the secretion of IL-1β, IL-10, IL-12, KC, MIP-1α, IFN-γ and IL-6 (p0.05). Treatment with CHX significantly increased secretion of IL-4 and RANTES (p0.05).with EGCG significantly increased Eotaxin secretion (p0.05). Both CHX and EGCG significantly decreased secretion of IL-17 (p0.05). GM-CSF and TNF-α did not present significant change in secretion after treatment with either CHX or EGCG (p0.05).Both CHX and EGCG modulate secretion of various inflammatory and anti-inflammatory mediators in odontoblastic cells. |
| Databáze: | OpenAIRE |
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