Sensitive high-performance liquid chromatographic method for the determination of chlorhexidine in human serum and urine
| Autor: | Haiyung Cheng, Linda R. Brougham, Kenneth A. Pittman |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Chloroform
Chromatography Aniline Compounds Chemistry medicine.drug_class Sodium Chlorhexidine chemistry.chemical_element General Chemistry Urine High-performance liquid chromatography Matrix (chemical analysis) chemistry.chemical_compound Antiseptic medicine Solvents Humans Indicators and Reagents Chromatography High Pressure Liquid medicine.drug Antibacterial agent |
| Zdroj: | Journal of chromatography. 383(2) |
| Popis: | A high-performance liquid chromatographic method for the analysis of chlorhexidine in human serum and urine was developed. Chlorhexidine and the internal standard, chlorpheniramine, were extracted into chloroform, containing 5% 2-propanol, and back-extracted into dilute sulfuric acid. Chromatographic separation was achieved on a C 18 column equilibrated with methanol—water (70:30, v/v), containing 0.005 M sodium heptane-sulfonate. The sensitivity of the assay was 20 ng/ml of biological matrix, using 0.5-ml samples. The application of this method to monitor chlorhexidine levels in burn patients treated topically with a chlorhexidine-containing burn cream was demonstrated. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|