Biofilm formation byBorrelia burgdorferisensu lato
| Autor: | David F. Luecke, Venkata Arun Timmaraju, Eva Sapi, Shafiq Shakih, Priyanka A. S. Theophilus, Kunthavai Balasubramanian |
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| Rok vydání: | 2015 |
| Předmět: |
Biology
Microscopy Atomic Force Borrelia afzelii medicine.disease_cause Microbiology Extracellular polymeric substance Lyme disease Borrelia burgdorferi Group Borrelia parasitic diseases Genetics medicine Borrelia burgdorferi Molecular Biology Lyme Disease Polysaccharides Bacterial Biofilm biochemical phenomena metabolism and nutrition bacterial infections and mycoses medicine.disease biology.organism_classification United States Europe Phenotype Biofilms Ultrastructure Borrelia garinii |
| Zdroj: | FEMS Microbiology Letters. 362:fnv120 |
| ISSN: | 1574-6968 |
| Popis: | Bacterial biofilms are microbial communities held together by an extracellular polymeric substance matrix predominantly composed of polysaccharides, proteins and nucleic acids. We had previously shown that Borrelia burgdorferi sensu stricto, the causative organism of Lyme disease in the United States is capable of forming biofilms in vitro. Here, we investigated biofilm formation by B. afzelii and B. garinii, which cause Lyme disease in Europe. Using various histochemistry and microscopy techniques, we show that B. afzelii and B. garinii form biofilms, which resemble biofilms formed by B. burgdorferi sensu stricto. High-resolution atomic force microscopy revealed similarities in the ultrastructural organization of the biofilms form by three Borrelia species. Histochemical experiments revealed a heterogeneous organization of exopolysaccharides among the three Borrelia species. These results suggest that biofilm formation might be a common trait of Borrelia genera physiology. |
| Databáze: | OpenAIRE |
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