Succinivibrionaceae is dominant family in fecal microbiota of Behçet's Syndrome patients with uveitis

Autor:	Murat Hasanreisoglu, K. S. Diker, Duygu Tecer, Merve Erdogan, Tarkan Karakan, Feride Gogus, Çağrı Ergin, Seda Coban, Ramazan Kozan, Ayse Kalkanci
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	0301 basic medicine Male genomic DNA Familial Mediterranean fever Disease Gut flora Pathogenesis Feces 0302 clinical medicine autoinflammatory disease familial Mediterranean fever Bacteroides innate immunity intestine flora clinical article Multidisciplinary azathioprine biology adult Behcet Syndrome steroid Crohn disease female mesalazine uveitis Medicine Female microbial community Vasculitis Uveitis Research Article feces microflora Adult RNA 16S Science HLA B51 antigen salazosulfapyridine complication gene sequence colchicine methotrexate Article 03 medical and health sciences medicine Humans controlled study cyclosporine human 030203 arthritis & rheumatology Innate immune system nonhuman semiconductor sequencing business.industry isolation and purification microbiology immunopathogenesis Succinivibrionaceae case control study medicine.disease biology.organism_classification DNA isolation human tissue 030104 developmental biology 6S rRNA gene Gram negative infection Immunology prognosis microbiological examination business infliximab Gram-Negative Bacterial Infections Behcet disease
Zdroj:	PLoS ONE, Vol 15, Iss 10, p e0241691 (2020) PLoS ONE
ISSN:	1932-6203
Popis:	Behçet’s Syndrome (BS) is a multisystem vasculitis with various clinical manifestations. Pathogenesis is unclear, but studies have shown genetic factors, innate immunity and autoinflammation to have an important role in the disease course. Diversity in the microbial community of gut microbiota may significantly contribute to the activation of the innate immune system. The clinical features of BS present themselves in clusters and each cluster may be a consequence of different disease mechanisms. For this reason we aimed to investigate the gut microbiota of BS patients with uveitis. In addition to healthy controls, we have aimed to compare the gut microbiota of BS with that of Familial Mediterranean Fever (FMF) and Crohn’s Disease (CD) as both diseases have innate and autoinflammatory features in their pathogenesis. Seven patients with BS, 12 patients with FMF, 9 patients with CD and 16 healthy controls (HC) were included in the study. Total genomic DNAs were isolated from fecal samples of the patients. Partial 16S rRNA gene was sequenced using the PGM Ion Torrent (Thermo Fisher Scientific, Waltham, MA, USA) for microbiota analysis. Statistical analysis showed that significant differences were detected on the microbial community of four groups. Succinivibrionaceae is dominant and the signature family, whereas Bacteroides was absent in BS patients. Copyright: © 2020 Tecer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Databáze:	OpenAIRE
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