Emetic toxin formation of Bacillus cereus is restricted to a single evolutionary lineage of closely related strains

Autor: Birgitta Svensson, Per Einar Granum, Christophe Nguyen-The, Mirja Salkinoja-Salonen, Maria A. Andersson, Anders Christiansson, Monika Ehling-Schulz, Martina Fricker, Toril Lindbäck, Erwin Märtlbauer, Anja Schulz, Siegfried Scherer, Marie-Hélène Guinebretière
Přispěvatelé: Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ProdInra, Migration
Rok vydání: 2005
Předmět:
Diarrhea
Bacterial Toxins
Molecular Sequence Data
Bacillus cereus
Virulence
Polymerase Chain Reaction
Microbiology
Evolution
Molecular
Foodborne Diseases
03 medical and health sciences
chemistry.chemical_compound
Bacterial Proteins
RAPD
Depsipeptides
Spectroscopy
Fourier Transform Infrared
Genotype
Humans
[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
ComputingMilieux_MISCELLANEOUS
Gram-Positive Bacterial Infections
030304 developmental biology
Genetics
0303 health sciences
biology
030306 microbiology
Sequence Analysis
DNA
Cereulide
biology.organism_classification
Haemolysis
Bacterial Typing Techniques
Random Amplified Polymorphic DNA Technique
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Cereus
chemistry
Food Microbiology
Multilocus sequence typing
SPECTROSCOPIE INFRAROUGE
Emetics
Zdroj: Microbiology
Microbiology, Microbiology Society, 2005, 151, pp.183-197
HAL
ISSN: 1465-2080
1350-0872
DOI: 10.1099/mic.0.27607-0
Popis: An in-depth polyphasic approach was applied to study the population structure of the human pathogen Bacillus cereus. To assess the intraspecific biodiversity of this species, which is the causative agent of gastrointestinal diseases, a total of 90 isolates from diverse geographical origin were studied by genetic [M13-PCR, random amplification of polymorphic DNA (RAPD), multilocus sequence typing (MLST)] and phenetic [Fourier transform Infrared (FTIR), protein profiling, biochemical assays] methods. The strain set included clinical strains, isolates from food remnants connected to outbreaks, as well as isolates from diverse food environments with a well documented strain history. The phenotypic and genotypic analysis of the compiled panel of strains illustrated a considerable diversity among B. cereus connected to diarrhoeal syndrome and other non-emetic food strains, but a very low diversity among emetic isolates. Using all typing methods, cluster analysis revealed a single, distinct cluster of emetic B. cereus strains. The isolates belonging to this cluster were neither able to degrade starch nor could they ferment salicin; they did not possess the genes encoding haemolysin BL (Hbl) and showed only weak or no haemolysis. In contrast, haemolytic-enterotoxin-producing B. cereus strains showed a high degree of heterogeneity and were scattered over different clusters when different typing methods were applied. These data provide evidence for a clonal population structure of cereulide-producing emetic B. cereus and indicate that emetic strains represent a highly clonal complex within a potentially panmictic or weakly clonal background population structure of the species. It may have originated only recently through acquisition of specific virulence factors such as the cereulide synthetase gene.
Databáze: OpenAIRE
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