Phenotype and genomic background of Arcobacter butzleri strains and taxogenomic assessment of the species
| Autor: | Daniele Chieffi, Anna Mottola, Angela Di Pinto, Federico Baruzzi, Francesca Fanelli, Vincenzina Fusco |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Virulence
Subspecies Microbiology Genome 03 medical and health sciences RNA Ribosomal 16S Vegetables Aliarcobacter butzleri Antibiotic and heavy metal resistance Shellfish 030304 developmental biology Arcobacter Genetics 0303 health sciences biology 030306 microbiology Arcobacter butzleri Computational Biology Genomics biology.organism_classification 16S ribosomal RNA Phenotype Multilocus sequence typing Lithotrophic bacteria Multilocus Sequence Typing Food Science |
| Zdroj: | Food microbiology 89 (2020). doi:10.1016/j.fm.2020.103416 info:cnr-pdr/source/autori:Fanelli F.; Chieffi D.; Di Pinto A.; Mottola A.; Baruzzi F.; Fusco V./titolo:Phenotype and genomic background of Arcobacter butzleri strains and taxogenomic assessment of the species/doi:10.1016%2Fj.fm.2020.103416/rivista:Food microbiology (Print)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:89 |
| ISSN: | 0740-0020 |
| DOI: | 10.1016/j.fm.2020.103416 |
| Popis: | In this study the phenotypic and genomic characterization of two Arcobacter butzleri (Ab) strains (Ab 34_O and Ab 39_O) isolated from pre-cut ready-to-eat vegetables were performed. Results provided useful data about their taxonomy and their overall virulence potential with particular reference to the antibiotic and heavy metal susceptibility. These features were moreover compared with those of two Ab strains isolated from shellfish and a genotaxonomic assessment of the Ab species was performed. The two Ab isolated from vegetables were confirmed to belong to the Aliarcobacter butzleri species by 16S rRNA gene sequence analysis, MLST and genomic analyses. The genome-based taxonomic assessment of the Ab species brought to the light the possibility to define different subspecies reflecting the source of isolation, even though further genomes from different sources should be available to support this hypothesis. The strains isolated from vegetables in the same geographic area shared the same distribution of COGs with a prevalence of the cluster “inorganic ion transport and metabolism”, consistent with the lithotrophic nature of Arcobacter spp. None of the Ab strains (from shellfish and from vegetables) metabolized carbohydrates but utilized organic acids and amino acids as carbon sources. The metabolic fingerprinting of Ab resulted less discriminatory than the genome-based approach. The Ab strains isolated from vegetables and those isolated from shellfish endowed multiple resistance to several antibiotics and heavy metals. |
| Databáze: | OpenAIRE |
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