Phenotypic and genotypic characteristics of methicillin/oxacillin-resistant Staphylococcus intermedius isolated from clinical specimens during routine veterinary microbiological examinations
| Autor: | Michael Zschöck, Ch. Lämmler, I.E.M. El Zubeir, J. Alber, T. Kanbar, Ömer Akineden, Reinhard Weiss |
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| Rok vydání: | 2007 |
| Předmět: |
DNA
Bacterial Veterinary medicine Penicillin Resistance Staphylococcus Population Microbial Sensitivity Tests Cat Diseases medicine.disease_cause Staphylococcal infections Polymerase Chain Reaction Microbiology law.invention Methicillin Dogs law Drug Resistance Multiple Bacterial Multiplex polymerase chain reaction medicine Pulsed-field gel electrophoresis Animals Dog Diseases education Polymerase chain reaction Oxacillin Antibacterial agent education.field_of_study General Veterinary biology Staphylococcus intermedius General Medicine Staphylococcal Infections biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Staphylococcus aureus Cats Methicillin Resistance |
| Zdroj: | Veterinary Microbiology. 121:170-176 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2006.11.014 |
| Popis: | Methicillin/oxacillin resistance of 10 S. intermedius strains was investigated by conventional and molecular methods. The strains tested had been isolated in Germany during routine veterinary microbiological examinations of specimens from a small animal clinic between May and September 2005. Epidemiological relationships of the strains were studied by macrorestriction analysis of their chromosomal DNA using pulsed field gel electrophoresis (PFGE). Species identity of the 10 S. intermedius strains was confirmed by conventional methods and by PCR mediated amplification of S. intermedius specific segments of thermonuclease encoding gene nuc. As controls, four methicillin/oxacillin resistant S. intermedius (MRSI) strains obtained from specimens sent by four veterinarians and three selected methicillin/oxacillin sensitive S. intermedius (MSSI), also obtained from the small animal clinic, were tested. The 10 strains, representing approximately 6% of all S. intermedius isolated from the clinic throughout the time period mentioned above, and the four MRSI obtained from veterinarians, were methicillin/oxacillin and penicillin resistant using disk diffusion tests and could be cultivated on oxacillin resistant screening agar base (ORSAB). Both resistances could be confirmed by multiplex PCR detecting the resistance genes mecA and blaZ. The three MSSI were methicillin/oxacillin sensitive in all tests. Epidemiological investigation by macrorestriction analysis of the chromosomal DNA of the strains by pulsed field gel electrophoresis revealed that all 10 MRSI strains obtained from the clinic and the four MRSI strains obtained from veterinarians, in contrast to the three MSSI strains, represent identical or closely related bacterial clones possibly indicating a cross-infection of the animals in the clinic and the distribution of a single MRSI clone in the pet population. |
| Databáze: | OpenAIRE |
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