Inhibition of initiation of DNA synthesis by aminoglycoside antibiotics
| Autor: | Keita Matsunaga, Toshio Nishimura, Hiroshi Yamaki, Nobuo Tanaka |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
DNA
Bacterial Dibekacin medicine.drug_class Antibiotics Mutant Biophysics Biology Biochemistry chemistry.chemical_compound Escherichia coli medicine Molecular Biology DNA synthesis Chloramphenicol Aminoglycoside Temperature Kanamycin Cell Biology Molecular biology Anti-Bacterial Agents Aminoglycosides chemistry Mutation DNA medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 122:460-465 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(84)90498-4 |
| Popis: | In a dnaCts mutant of E. coli, the reinitiation of DNA synthesis, which occurred by the shift of the culture from a restrictive temperature to a permissive temperature, was markedly prevented by habakacin, dibekacin, kanamycin, and gentamicin. On the contrary, chloramphenicol did not inhibit the reinitiation synthesis for 30 min. In a parallel experiment, leucine uptake into protein was profoundly blocked by chloramphenicol, but only slightly by habekacin. Habekacin did not significantly affect DNA elongation of the cells at a restrictive temperature. We propose that inhibition of initiation of replication by aminoglycoside antibiotics is related to their lethality. |
| Databáze: | OpenAIRE |
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