An in vitro coculture model of transmigrant monocytes and foam cell formation
| Autor: | Etsuo Niki, Noriko Noguchi, Yoshio Yazaki, Yoko Kumazawa, Michihisa Umetani, Motohiro Takeya, Hiroki Kurihara, Tatsuhiko Kodama, Mikio Takaku, Youichiro Wada, Yuko Okimoto, Takao Hamakubo, Kiyoshi Takahashi, Katsunori Jinnouchi, Makoto Naito, Hiroyuki Usuda |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Silver Staining Arteriosclerosis Cell Culture Techniques Motility Biology Monocytes Muscle Smooth Vascular Cell Movement medicine Macrophage Animals Humans Scavenger receptor Aorta Chemokine CCL2 Foam cell Monocyte Chemotaxis In vitro Cell biology Lipoproteins LDL medicine.anatomical_structure Biochemistry Cell culture Microscopy Electron Scanning Silver Nitrate Collagen Rabbits Cardiology and Cardiovascular Medicine Foam Cells |
| Zdroj: | Arteriosclerosis, thrombosis, and vascular biology. 19(10) |
| ISSN: | 1079-5642 |
| Popis: | Abstract —To analyze in vitro the migration of monocytes to the subendothelial space, their differentiation into macrophages, and the subsequent formation of foam cells in vitro, we have developed a 2-coculture system with rabbit aortic endothelial cells (AECs), aortic smooth muscle cells (SMCs), and a mixture of matrix proteins on polyethylene filters in chemotaxis chambers. AECs were seeded on a mixture of type I and IV collagen with or without various types of serum lipoproteins (method 1) or on matrix proteins secreted by SMCs (method 2). In these coculture systems, rabbit AECs can maintain a well-preserved monolayer for up to 2 weeks. When human CD14-positive monocytes were added to the upper medium of the system, with monocyte chemotactic protein-1 treatment ≈60% of the monocytes transmigrated within 24 hours and were retained for up to 7 days, whereas without MCP-1 treatment |
| Databáze: | OpenAIRE |
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