CD27 expression in the human splenic marginal zone: the infant marginal zone is populated by naive B cells
| Autor: | Andre Zandvoort, N. K. De Boer, F. G. M. Kroese, Wim Timens, Monique E. Lodewijk, Peter M. Dammers |
|---|---|
| Přispěvatelé: | Groningen Research Institute for Asthma and COPD (GRIAC), Translational Immunology Groningen (TRIGR), Guided Treatment in Optimal Selected Cancer Patients (GUTS) |
| Rok vydání: | 2002 |
| Předmět: |
Adult
ANTIBODY-RESPONSES Adolescent HUMAN SPLEEN medicine.drug_class CD3 Immunology Naive B cell Gene Expression chemical and pharmacologic phenomena Spleen Monoclonal antibody PNEUMOCOCCAL POLYSACCHARIDE Biochemistry Immunoglobulin D Immune system Antigen Genetics medicine IMMUNE-RESPONSE Immunology and Allergy Humans human CD27 B cells B-Lymphocytes biology VARIABLE REGION GENES Infant Newborn Infant IN-VITRO General Medicine Marginal zone Lymphocyte Subsets TI-2 ANTIGENS Tumor Necrosis Factor Receptor Superfamily Member 7 medicine.anatomical_structure CD27/CD70 INTERACTION Child Preschool T-CELLS biology.protein marginal zone Receptors Complement 3d LYMPHOID-TISSUES Immunologic Memory |
| Zdroj: | TISSUE ANTIGENS, 58(4), 234-242. BLACKWELL PUBLISHING |
| ISSN: | 0001-2815 |
| Popis: | The splenic marginal zone of adult humans contains B cells, of which most express CD27, an antigen only recently identified as a marker for somatically, mutated memory B cells. We investigated whether and to which extent the developing marginal zone in infants arid children is populated by either memory (CD27+) or naive (CD27-) B cells. Frozen sections of 32 spleens of infants and children ranging in age from 6 days to 15 years and 6 adult spleens were investigated. The expression of CD27 in combination with monoclonal antibodies against CD3, CD21, IgM, IgD and ASM. 1 was analyzed by immunohistochemistry. The marginal zone was already present at 4 months after birth but CD21 expression was observed first after 2 years. CD27-positive marginal zone B cells were observed firstly 2 years after birth and increased in number to adult levels at the age of 5 years. We demonstrated that the MZ of infants and young children is populated by naive B cells, which are replaced by memory B cells in a time frame of 2 to 5 years. Before the age of 2 years, although present, memory B cells appear to be unable to colonize the marginal zone, Because of the absence of memory B cells in the marginal zone, the immune system of a child is not capable to initiate a rapid secondary humoral immune response comparable to the adult immune response. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text