Parathyroid hormone-related peptide and 8701-BC breast cancer cell growth and invasion in vitro: evidence for growth-inhibiting and invasion-promoting effects
| Autor: | William J. Burtis, Claudio Luparello, Friedhelm Raue, M.A. Birch, James A. Gallagher |
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| Rok vydání: | 1995 |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty Cell division Molecular Sequence Data Parathyroid hormone Breast Neoplasms Biology Polymerase Chain Reaction Biochemistry Endocrinology Internal medicine Endopeptidases Tumor Cells Cultured medicine Extracellular Humans Neoplasm Invasiveness Protease Inhibitors RNA Messenger Receptor Molecular Biology Receptor Parathyroid Hormone Type 1 Base Sequence Parathyroid hormone-related protein Cell growth Parathyroid Hormone-Related Protein Proteolytic enzymes Proteins RNA-Directed DNA Polymerase In vitro Parathyroid Hormone Cancer research Receptors Parathyroid Hormone Cell Division hormones hormone substitutes and hormone antagonists |
| Zdroj: | Molecular and Cellular Endocrinology. 111:225-232 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/0303-7207(95)03577-t |
| Popis: | It has been previously reported that 8701-BC cells, derived from a primary carcinoma of the breast, constitutively express parathyroid hormone-related peptide (PTHrP) gene and that N-terminal PTHrP immunoreactivity can be found in cell medium. Here we have firstly measured immunoreactive PTHrP in 8701-BC cell medium using antibodies raised against midregion and C-terminal fragments, and also demonstrated the expression of PTH/PTHrP receptor by 8701-BC cells. Secondly, we have examined the role, if any, elicited by diverse PTHrP domains on 8701-BC cell proliferation, and invasive behaviour in vitro related to production of extracellular proteolytic enzymes. Our data show that PTHrP [1-34], and, to a minor extent, [67-86] and [107-139], are anti-mitogenic but 'invadogenic' for 8701-BC cells, and suggest that diverse enzymatic activities may contribute to cell invasion in response to different PTHrP fragments. In light of the present data on a chemoattractive role for PTHrP in vitro, we hypothesize that this protein might intervene in local control of the invasive process in breast carcinoma. |
| Databáze: | OpenAIRE |
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