Traumatic brain injury impairs sensorimotor function in mice
| Autor: | Laurel Haines, Adrian M. Sackheim, Sheila Russell, Sayamwong E. Hammack, Chary L. Scott, David W. Stockwell, Kalev Freeman, Nuria Villalba |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Reflex Startle medicine.medical_specialty Startle response Scoring system Traumatic brain injury Neuropsychological Tests Article Mice Random Allocation 03 medical and health sciences Injury Severity Score 0302 clinical medicine Physical medicine and rehabilitation Brain Injuries Traumatic Animals Medicine Postural Balance Balance (ability) Neurologic Examination Observer Variation medicine.diagnostic_test business.industry Sham surgery Magnetic resonance imaging medicine.disease Magnetic Resonance Imaging Mice Inbred C57BL 030104 developmental biology Fluid percussion Anesthesia Surgery business 030217 neurology & neurosurgery |
| Zdroj: | Journal of Surgical Research. 213:100-109 |
| ISSN: | 0022-4804 |
| Popis: | Introduction Understanding the extent to which murine models of traumatic brain injury (TBI) replicate clinically relevant neurologic outcomes is critical for mechanistic and therapeutic studies. We determined sensorimotor outcomes in a mouse model of TBI and validated the use of a standardized neurologic examination scoring system to quantify the extent of injury. Materials and methods We used a lateral fluid percussion injury model of TBI and compared TBI animals to those that underwent sham surgery. We measured neurobehavioral deficits using a standardized 12-point neurologic examination, magnetic resonance imaging, a rotating rod test, and longitudinal acoustic startle testing. Results TBI animals had a significantly decreased ability to balance on a rotating rod and a marked reduction in the amplitude of acoustic startle response. The neurologic examination had a high inter-rater reliability (87% agreement) and correlated with latency to fall on a rotating rod (R s = −0.809). Conclusions TBI impairs sensorimotor function in mice, and the extent of impairment can be predicted by a standardized neurologic examination. |
| Databáze: | OpenAIRE |
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