Unbiased phosphoproteomic method identifies the initial effects of a methacrylic acid copolymer on macrophages
| Autor: | Michael V. Sefton, Alexandra Lisovsky, Andrew Emili, Hongbo Guo, Ilana Talior-Volodarsky, Michael Dean Chamberlain, Redouan Mahou, Laura A. Wells, Ruth Isserlin |
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| Rok vydání: | 2015 |
| Předmět: |
Proteomics
Materials science Integrin Biocompatible Materials Methylmethacrylate chemistry.chemical_compound Polymethacrylic Acids Cell Line Tumor Materials Testing Phosphoprotein Phosphatases Humans Phosphorylation Methyl methacrylate skin and connective tissue diseases Cytoskeleton Multidisciplinary biology Gene Expression Profiling Macrophages Intracellular Signaling Peptides and Proteins Membrane Proteins Biological Sciences Phosphoproteins Neoplasm Proteins Cell biology Cytoskeletal Proteins Methacrylic acid chemistry Membrane protein Biochemistry Apoptosis biology.protein sense organs Adsorption Apoptosis Regulatory Proteins Cell Adhesion Molecules Protein Kinases Protein Processing Post-Translational Transcription Factors |
| Zdroj: | Proceedings of the National Academy of Sciences. 112:10673-10678 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1508826112 |
| Popis: | An unbiased phosphoproteomic method was used to identify biomaterial-associated changes in the phosphorylation patterns of macrophage-like cells. The phosphorylation differences between differentiated THP1 (dTHP1) cells treated for 10, 20, or 30 min with a vascular regenerative methacrylic acid (MAA) copolymer or a control methyl methacrylate (MM) copolymer were determined by MS. There were 1,470 peptides (corresponding to 729 proteins) that were differentially phosphorylated in dTHP1 cells treated with the two materials with a greater cellular response to MAA treatment. In addition to identifying pathways (such as integrin signaling and cytoskeletal arrangement) that are well known to change with cell-material interaction, previously unidentified pathways, such as apoptosis and mRNA splicing, were also discovered. |
| Databáze: | OpenAIRE |
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