The SARS-unique domain (SUD) of SARS coronavirus contains two macrodomains that bind G-quadruplexes
| Autor: | Clemens Vonrhein, Gérard Bricogne, Christian L. Schmidt, Oliver S. Smart, Rolf Hilgenfeld, Guido Hansen, Jinzhi Tan, Yuri Kusov, Michela Bollati, Jeroen R. Mesters |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Protein Folding
Protein Conformation viruses Biophysics/Protein Folding RNA-binding protein Plasma protein binding Viral Nonstructural Proteins Crystallography X-Ray Virus Replication medicine.disease_cause Biochemistry Protein structure Virology/Virulence Factors and Mechanisms Peptide sequence lcsh:QH301-705.5 Mutation humanities Severe acute respiratory syndrome-related coronavirus Virology/Viral Replication and Gene Regulation Protein Binding Research Article Electrophoresis lcsh:Immunologic diseases. Allergy Molecular Sequence Data Immunology Protein domain Biophysics RNA-dependent RNA polymerase Genome Viral Biology Microbiology behavioral disciplines and activities Virology/Emerging Viral Diseases Virology mental disorders Genetics medicine Biochemistry/RNA Structure Amino Acid Sequence Molecular Biology Adenosine Diphosphate Ribose Lysine Biochemistry/Structural Genomics Biophysics/Structural Genomics RNA-Dependent RNA Polymerase Molecular biology Protein Structure Tertiary Biophysics/RNA Structure G-Quadruplexes Viral replication lcsh:Biology (General) Parasitology Protein Multimerization lcsh:RC581-607 |
| Zdroj: | PLoS Pathogens, Vol 5, Iss 5, p e1000428 (2009) PLoS Pathogens |
| ISSN: | 1553-7374 1553-7366 |
| Popis: | Since the outbreak of severe acute respiratory syndrome (SARS) in 2003, the three-dimensional structures of several of the replicase/transcriptase components of SARS coronavirus (SARS-CoV), the non-structural proteins (Nsps), have been determined. However, within the large Nsp3 (1922 amino-acid residues), the structure and function of the so-called SARS-unique domain (SUD) have remained elusive. SUD occurs only in SARS-CoV and the highly related viruses found in certain bats, but is absent from all other coronaviruses. Therefore, it has been speculated that it may be involved in the extreme pathogenicity of SARS-CoV, compared to other coronaviruses, most of which cause only mild infections in humans. In order to help elucidate the function of the SUD, we have determined crystal structures of fragment 389–652 (“SUDcore”) of Nsp3, which comprises 264 of the 338 residues of the domain. Both the monoclinic and triclinic crystal forms (2.2 and 2.8 Å resolution, respectively) revealed that SUDcore forms a homodimer. Each monomer consists of two subdomains, SUD-N and SUD-M, with a macrodomain fold similar to the SARS-CoV X-domain. However, in contrast to the latter, SUD fails to bind ADP-ribose, as determined by zone-interference gel electrophoresis. Instead, the entire SUDcore as well as its individual subdomains interact with oligonucleotides known to form G-quadruplexes. This includes oligodeoxy- as well as oligoribonucleotides. Mutations of selected lysine residues on the surface of the SUD-N subdomain lead to reduction of G-quadruplex binding, whereas mutations in the SUD-M subdomain abolish it. As there is no evidence for Nsp3 entering the nucleus of the host cell, the SARS-CoV genomic RNA or host-cell mRNA containing long G-stretches may be targets of SUD. The SARS-CoV genome is devoid of G-stretches longer than 5–6 nucleotides, but more extended G-stretches are found in the 3′-nontranslated regions of mRNAs coding for certain host-cell proteins involved in apoptosis or signal transduction, and have been shown to bind to SUD in vitro. Therefore, SUD may be involved in controlling the host cell's response to the viral infection. Possible interference with poly(ADP-ribose) polymerase-like domains is also discussed. Author Summary The genome of the SARS coronavirus codes for 16 non-structural proteins that are involved in replicating this huge RNA (approximately 29 kilobases). The roles of many of these in replication (and/or transcription) are unknown. We attempt to derive conclusions concerning the possible functions of these proteins from their three-dimensional structures, which we determine by X-ray crystallography. Non-structural protein 3 contains at least seven different functional modules within its 1922-amino-acid polypeptide chain. One of these is the so-called SARS-unique domain, a stretch of about 338 residues that is completely absent from any other coronavirus. It may thus be responsible for the extraordinarily high pathogenicity of the SARS coronavirus, compared to other viruses of this family. We describe here the three-dimensional structure of the SARS-unique domain and show that it consists of two modules with a known fold, the so-called macrodomain. Furthermore, we demonstrate that these domains bind unusual nucleic-acid structures formed by consecutive guanosine nucleotides, where four strands of nucleic acid are forming a superhelix (so-called G-quadruplexes). SUD may be involved in binding to viral or host-cell RNA bearing this peculiar structure and thereby regulate viral replication or fight the immune response of the infected host cell. |
| Databáze: | OpenAIRE |
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