The expression pattern, polymorphisms and association analyses of the porcine NREP gene

Autor: Michaela Nesvadbová, T. Urban, Aleš Knoll
Rok vydání: 2021
Předmět:
Zdroj: Journal of Animal Breeding and Genetics. 139:62-70
ISSN: 1439-0388
0931-2668
DOI: 10.1111/jbg.12646
Popis: NREP (neuronal regeneration related protein homolog) plays a role in the transformation of neural, muscle, and fibroblast cells and in smooth muscle myogenesis. The NREP gene was selected for detailed study as an expressional and functional candidate gene on the basis of data from the expression microarray, which detected the differences in gene expression between Czech Large White pigs and wild boars in the longissimus lumborum et thoracis and biceps femoris muscle tissues. Quantitative real-time PCR results confirmed that porcine NREP was expressed in both skeletal muscles and significantly overexpressed in Czech Large White pigs compared with wild boars (14.5- and 11.6-fold; p .05). We identified 9 polymorphic sites in the genomic DNA of NREP. Six of these polymorphisms were in complete linkage disequilibrium, and therefore, only 4 loci were informative. The associations of the HF571253:g.103GA, HF571253:g.134GA, HF571253:g.179TC and HF571253:g.402_409delT polymorphisms with backfat thickness, lean meat content and average daily gain were assessed in Czech Large White pigs. The GG genotypes HF571253:g.103GA and HF571253:g.134GA, the TT genotypes HF571253:g.179TC and 67 HF571253:g.402_409delT genotypes had favourable effects on the studied traits. Our results indicate the possibility of utilizing the variability of the NREP gene in marker-assisted selection in order to improve meat production in pigs.
Databáze: OpenAIRE
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