Comparison of Gold Nanoparticle Conjugated Secondary Antibody with Non-Gold Secondary Antibody in an ELISA Kit Model
| Autor: | R. Madani, F. Golchinfar, Seyed Mohammad Amini, Abdolhamid Shoushtary, Tara Emami |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
animal structures
medicine.drug_class Immunology Nanoparticle Metal Nanoparticles Enzyme-Linked Immunosorbent Assay Monoclonal antibody Antibodies Absorbance medicine Immunology and Allergy Animals Peroxidase Drug Carriers Chromatography biology Chemistry Substrate (chemistry) Molecular biology Primary and secondary antibodies Colloidal gold Influenza in Birds biology.protein Gold Drug carrier Chickens |
| Zdroj: | Monoclonal antibodies in immunodiagnosis and immunotherapy. 34(5) |
| ISSN: | 2167-9436 |
| Popis: | In this study, gold nanoparticles (AuNPs) were used as carriers of the signaling anti-chicken antibody peroxidase in comparison with anti-chicken antibody peroxidase without gold nanoparticle in a commercial avian influenza kit. AuNPs enhanced the absorbance and shortened the assay time. AuNPs act as a carrier of many enzymes and multiply the effect of enzyme when reacting with substrate. They amplify optical signal, while keeping low background signals. |
| Databáze: | OpenAIRE |
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