Aldehyde dehydrogenase (ALDH) 2 associates with oxidation of methoxyacetaldehyde; in vitro analysis with liver subcellular fraction derived from human andAldh2gene targeting mouse
| Autor: | Akira Yoshida, Toshihiro Kawamoto, Keiichi I. Nakayama, Naoki Kunugita, Keiko Nakayama, Tadasuke Tsukiyama, Kyoko Kitagawa, Kohji Okamoto |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Genotype Mutant Biophysics Aldehyde dehydrogenase Acetaldehyde Oxidative phosphorylation In Vitro Techniques Biochemistry Mice Structural Biology Genetics Animals Humans Molecular Biology Alleles 2-Methoxyethanol DNA Primers ALDH2 Mice Knockout Gene targeting mouse chemistry.chemical_classification Polymorphism Genetic Base Sequence biology Aldehyde Dehydrogenase Mitochondrial Wild type Cell Biology Metabolism Aldehyde Dehydrogenase Molecular biology Methoxyacetaldehyde Mice Inbred C57BL Enzyme Liver chemistry Aldehyde dehydrogenase 2 Knockout mouse biology.protein Female Oxidation-Reduction Subcellular Fractions |
| Zdroj: | FEBS Letters. 476:306-311 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(00)01710-5 |
| Popis: | A principal pathway of 2-methoxyethanol (ME) metabolism is to the toxic oxidative product, methoxyacetaldehyde (MALD). To assess the role of aldehyde dehydrogenase (ALDH) in MALD metabolism, in vitro MALD oxidation was examined with liver subcellular fractions from Japanese subjects who carried three different ALDH2 genotypes and Aldh2 knockout mice, which were generated in this study. The activity was distributed in mitochondrial fractions of ALDH2*1/*1 and wild type (Aldh2+/+) mice but not ALDH2*1/*2, *2/*2 subjects or Aldh2 homozygous mutant (Aldh2−/−) mice. These data suggest that ALDH2 is a key enzyme for MALD oxidation and ME susceptibility may be influenced by the ALDH2 genotype. |
| Databáze: | OpenAIRE |
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