A Rat Model of Central Venous Catheter to Study Establishment of Long-Term Bacterial Biofilm and Related Acute and Chronic Infections
| Autor: | David Lebeaux, Jean-Marc Ghigo, Irène Kriegel, Ashwini Chauhan, Marie-Christine Escande, Benoit Decante, Christophe Beloin |
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| Přispěvatelé: | Génétique des Biofilms, Institut Pasteur [Paris], Centre chirurgical Marie Lannelongue, Institut Curie [Paris], This work was financially supported by the PTR 279/PIC Institut Curie Infections Nosocomiales. David Lebeaux was supported by a grant from the AXA Research Fund., Institut Pasteur [Paris] (IP), Centre Chirurgical Marie Lannelongue (CCML) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Applied Microbiology Cefazolin lcsh:Medicine medicine.disease_cause MESH: Catheterization MESH: Bacterial biofilms MESH: Biofilms/*growth & development Catheters Indwelling MESH: Animal Staphylococcus epidermidis MESH: Models MESH: Staphylococcus aureus Gram Negative MESH: Animals MESH: Anti-Bacterial Agents/therapeutic use lcsh:Science Escherichia coli Infections Staphylococci MESH: Escherichia coli Infections/complications Escherichia Coli 0303 health sciences Multidisciplinary biology Animal Models Staphylococcal Infections MESH: Gentamicins/therapeutic use MESH: Escherichia coli infections Anti-Bacterial Agents Bacterial Pathogens 3. Good health Host-Pathogen Interaction Medical Microbiology Staphylococcus aureus Models Animal Pseudomonas aeruginosa MESH: Pseudomonas aeruginosa Gentamicin Research Article medicine.drug MESH: Central Venous/*adverse effects Catheterization Central Venous MESH: Rats MESH: Staphylococcus epidermidis Staphylococcal infections Microbiology MESH: *Equipment Contamination 03 medical and health sciences Model Organisms In vivo MESH: Immunosuppression/adverse effects Microbial Control MESH: Cefazolin/therapeutic use medicine Animals Pseudomonas Infections Biology Microbial Pathogens 030304 developmental biology MESH: Staphylococcal Infections/complications/drug therapy MESH: bacterial pathogens Immunosuppression Therapy 030306 microbiology lcsh:R Biofilm Bacteriology MESH: Indwelling/*adverse effects biology.organism_classification medicine.disease [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Male Rats MESH: Pseudomonas Infections/complications Biofilms Immunology Equipment Contamination Rat lcsh:Q Gentamicins MESH: Catheters Bacterial Biofilms MESH: Bloodstream infections |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2012, 7 (5), pp.e37281. ⟨10.1371/journal.pone.0037281⟩ PLoS ONE, Vol 7, Iss 5, p e37281 (2012) PLoS ONE, 2012, 7 (5), pp.e37281. ⟨10.1371/journal.pone.0037281⟩ |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0037281⟩ |
| Popis: | International audience; Formation of resilient biofilms on medical devices colonized by pathogenic microorganisms is a major cause of health-care associated infection. While in vitro biofilm analyses led to promising anti-biofilm approaches, little is known about their translation to in vivo situations and on host contribution to the in vivo dynamics of infections on medical devices. Here we have developed an in vivo model of long-term bacterial biofilm infections in a pediatric totally implantable venous access port (TIVAP) surgically placed in adult rats. Using non-invasive and quantitative bioluminescence, we studied TIVAP contamination by clinically relevant pathogens, Escherichia coli , Pseudomonas aeruginosa , Staphylococcus aureus and Staphylococcus epidermidis , and we demonstrated that TIVAP bacterial populations display typical biofilm phenotypes. In our study, we showed that immunocompetent rats were able to control the colonization and clear the bloodstream infection except for up to 30% that suffered systemic infection and death whereas none of the immunosuppressed rats survived the infection. Besides, we mimicked some clinically relevant TIVAP associated complications such as port-pocket infection and hematogenous route of colonization. Finally, by assessing an optimized antibiotic lock therapy, we established that our in vivo model enables to assess innovative therapeutic strategies against bacterial biofilm infections. |
| Databáze: | OpenAIRE |
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