Numerous potentially functional but non-genic conserved sequences on human chromosome 21
| Autor: | Stylianos E. Antonarakis, C. Victor Jongeneel, Samuel Deutsch, Volker Flegel, Philipp Bucher, Nathalie Scamuffa, Brian Stevenson, Emmanouil T. Dermitzakis, Catherine Ucla, Robert Lyle, Alexandre Reymond |
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| Rok vydání: | 2002 |
| Předmět: |
RNA
Untranslated/genetics Genomics Computational biology Biology Synteny Genome Conserved sequence Conserved non-coding sequence Mice/ genetics Animals Humans Genes/genetics Oligonucleotide Array Sequence Analysis Transcription Genetic/genetics ddc:616 Comparative genomics Genetics Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction Conserved Sequence/ genetics Computational Biology Pseudogenes/genetics Genome project Regulatory Sequences Nucleic Acid/genetics Chromosomes Human Pair 21/ genetics Rabbits Chromosome 21 Sequence Alignment |
| Zdroj: | Nature, Vol. 420, No 6915 (2002) pp. 578-582 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/nature01251 |
| Popis: | The use of comparative genomics to infer genome function relies on the understanding of how different components of the genome change over evolutionary time. The aim of such comparative analysis is to identify conserved, functionally transcribed sequences such as protein-coding genes and non-coding RNA genes, and other functional sequences such as regulatory regions, as well as other genomic features. Here, we have compared the entire human chromosome 21 with syntenic regions of the mouse genome, and have identified a large number of conserved blocks of unknown function. Although previous studies have made similar observations, it is unknown whether these conserved sequences are genes or not. Here we present an extensive experimental and computational analysis of human chromosome 21 in an effort to assign function to sequences conserved between human chromosome 21 (ref. 8) and the syntenic mouse regions. Our data support the presence of a large number of potentially functional non-genic sequences, probably regulatory and structural. The integration of the properties of the conserved components of human chromosome 21 to the rapidly accumulating functional data for this chromosome will improve considerably our understanding of the role of sequence conservation in mammalian genomes. |
| Databáze: | OpenAIRE |
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