Prostaglandin synthesis by human amnion is dependent upon extracellular calcium
| Autor: | John R. G. Challis, Mary Anne Opavsky, David M. Olson |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
medicine.medical_specialty
Physiology Prostaglandin chemistry.chemical_element In Vitro Techniques Biology Calcium Calcium Chloride chemistry.chemical_compound Biosynthesis Pregnancy Physiology (medical) Internal medicine medicine Extracellular Humans Channel blocker Amnion Gallopamil Pharmacology General Medicine Metabolism EGTA Endocrinology medicine.anatomical_structure chemistry Prostaglandins Female Extracellular Space |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 61:1089-1092 |
| ISSN: | 1205-7541 0008-4212 |
| Popis: | Prostaglandin (PG) E and F output was studied in collagenase-dispersed amnion cells to determine the effect of extracellular Ca2+ upon PG synthesis. In the presence of 2.5 mM CaCl2, PGE and PGF output (picograms per 105 cells per 3 h) by cells obtained at term prior to labour following elective cesarean section (CS) was 183 ± 39 and 127 ± 23, respectively. This increased to 435 ± 111 (p 2 from the medium (plus 0.1 mM EGTA) significantly reduced (p 2 concentrations in the medium were decreased from 2.5 to 0.25 mM, but significantly attenuated (p 2 was decreased from 0.25 to 0 mM. The voltage-sensitive Ca2+ channel blocker, D-600, decreased PGE output in the presence of (2.5 mM) CaCl2 to levels observed in the absence of CaCl2. Ionophore A23187 restored PGE output in the presence of D-600 and Ca2+. PGF output from CS amnion cells was stimulated by A23187 and elevated extracellular K+ (40 mM). In each case, exclusion of CaCl2 from the medium eliminated the response. These, results suggest that PG output by human amnion is dependent, in part, upon the presence of extracellular Ca2+ and that Ca2+ may enter the cell via a potential-sensitive mechanism. |
| Databáze: | OpenAIRE |
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