Longitudinal detection of somatic mutations in saliva and plasma for the surveillance of oral squamous cell carcinomas
| Autor: | In Ho Cha, Jung Ah Park, Ju Yeong Park, Dawool Han, Hyungjun Kim, Ying Cui, Yong Hoon Cha, Hae-Suk Kim, Woong Nam, Eunae Sandra Cho |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Oncology Saliva Physiology Somatic cell Biopsy Cancer Treatment Circulating Tumor DNA Surgical pathology Medicine and Health Sciences Longitudinal Studies Multidisciplinary medicine.diagnostic_test Middle Aged Body Fluids Carcinoma Squamous Cell Medicine Female Mouth Neoplasms Anatomy Research Article medicine.medical_specialty Science Surgical and Invasive Medical Procedures Malignant Tumors Germline mutation Internal medicine Biomarkers Tumor Genetics medicine Humans Liquid biopsy Aged business.industry Head and neck cancer Liquid Biopsy Biology and Life Sciences Cancers and Neoplasms Cancer medicine.disease Head and Neck Cancers Mutation Somatic Mutation Neoplasm Recurrence Local business |
| Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 9, p e0256979 (2021) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0256979 |
| Popis: | Purposes Although clinical and radiological examinations can be used to diagnose oral cancer, and surgical pathology remains the gold standard, these conventional methods have limitations. We evaluated the feasibility of longitudinal next-generation sequencing-based liquid biopsy for oral squamous cell carcinoma surveillance. Materials and methods Eleven patients were enrolled, and plasma and saliva were collected before, and 1, 3, and 6 months after surgery. Tumor-specific mutations were selected using paired, whole-exome analyses of tumor tissues and whole blood. Genes frequently mutated in head and neck cancer were identified using the Cancer Genome Atlas (TCGA) and Catalogue of Somatic Mutations in Cancer (COSMIC) databases to design targeted deep sequencing panels. Results In five of the six patients with recurrent cancer, circulating tumor DNA (ctDNA) was detected earlier with liquid biopsy than with conventional monitoring techniques. Moreover, patients without recurrence exhibited decreased ctDNA allele frequency post-treatment. Conclusions Longitudinal liquid biopsy of plasma and saliva may be feasible for detecting somatic mutations associated with oral squamous cell carcinomas. It might be attributable to determine early tumor recurrence through genetic analysis of ctDNA. |
| Databáze: | OpenAIRE |
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