Chondro-inductive hyaluronic acid/chitosan coacervate-based scaffolds for cartilage tissue engineering
| Autor: | Gamze Torun Kose, Ozge Karabiyik Acar, A. Basak Kayitmazer, Seden Bedir |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cartilage
Articular Biochemistry Chitosan chemistry.chemical_compound Structural Biology Hyaluronic acid Animals Hyaluronic Acid Molecular Biology Aggrecan Cartilage oligomeric matrix protein Coacervate Tissue Engineering Tissue Scaffolds biology Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells General Medicine Chondrogenesis Rats Cell biology biology.protein Stem cell |
| Zdroj: | International Journal of Biological Macromolecules. 188:300-312 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2021.07.176 |
| Popis: | Injuries related to articular cartilage are among the most challenging musculoskeletal problems because of poor repair capacity of this tissue. The lack of efficient treatments for chondral defects has stimulated research on cartilage tissue engineering applications combining porous biocompatible scaffolds with stem cells in the presence of external stimuli. This work presents the role of rat bone marrow mesenchymal stem cell (BMSC) encapsulated-novel three-dimensional (3D) coacervate scaffolds prepared through complex coacervation between different chitosan salts (CHI) and sodium hyaluronate (HA). The 3D architecture of BMSC encapsulated scaffolds (HA/CHI) was shown by scanning electron microscopy (SEM) to have an interconnected structure to allow cell-cell and cell-matrix interactions. Chondrogenic induction of encapsulated BMSCs within HA/CHI coacervates demonstrated remarkable cellular viability in addition to the elevated expression levels of chondrogenic markers such as sex determining region Y-box 9 protein (SOX9), aggrecan (ACAN), cartilage oligomeric matrix protein (COMP) and collagen type II (COL2A1) by immunofluorescence staining, qPCR and ELISA test. Collectively, HA/CHI coacervates are promising candidates for future use of these scaffolds in cartilage tissue engineering applications. |
| Databáze: | OpenAIRE |
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