Generating a Fractal Microstructure of Laminin-111 to Signal to Cells
| Autor: | Tatiana Coelho-Sampaio, Camila Hochman-Mendez, Ariel J Kent, Claire Robertson, Jamie L. Inman, Mina J. Bissell |
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| Rok vydání: | 2020 |
| Předmět: |
General Immunology and Microbiology
Chemistry General Chemical Engineering General Neuroscience Microstructure Signal General Biochemistry Genetics and Molecular Biology Laminin 111 Article Extracellular Matrix Polymerization Extracellular matrix Fractal Fractals Biophysics Neural system Laminin Signal transduction Signal Transduction |
| Zdroj: | J Vis Exp |
| ISSN: | 1940-087X |
| Popis: | Laminin-111 (Ln1) is an essential part of the extracellular matrix in epithelia, muscle and neural systems. We have previously demonstrated that the microstructure of Ln1 alters the way that it signals to cells, possibly because Ln1 assembly into networks exposes different adhesive domains. In this protocol, we describe three methods to generate polymerized Ln1. |
| Databáze: | OpenAIRE |
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