Selective targeting of green fluorescent nanodiamond conjugates to mitochondria in HeLa cells
| Autor: | Tatiana Mikhailovna Gubarevich, Andrea Pohl, Jürgen Schreiber, Msaukiranji Mkandawire, Gerhard Rödel, Dietmar Appelhans, Jörg Opitz, Wolfgang Pompe, Victoria Alkseyevna Lapina |
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| Přispěvatelé: | Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Dendrimers
Immunoconjugates Spectrophotometry Infrared Surface Properties Static Electricity General Physics and Astronomy Molecular Probe Techniques Succinimides General Biochemistry Genetics and Molecular Biology HeLa Mitochondrial Proteins Fluorescence microscope Humans General Materials Science Cationic liposome Protamines Particle Size Nanodiamond Fluorescent Dyes biology Chemistry General Engineering Antibodies Monoclonal General Chemistry Transfection biology.organism_classification Fluorescence Actins Cell biology Mitochondria Autofluorescence Carbodiimides Spectrometry Fluorescence Microscopy Fluorescence Liposomes Nanoparticles Diamond Intracellular HeLa Cells |
| Popis: | Fluorescent cellular biomarkers play a prominent role in biosciences. Most of the available biomarkers have some drawbacks due to either physical and optical or cytotoxic properties. In view of this, we investigated the potential of green fluorescent nanodiamonds as biomarkers in living cells. Nanodiamonds were functionalized by attaching antibodies that target intracellular structures such as actin filaments and mitochondria. Then, the nanodiamond conjugates were transfected into HeLa cells. Transfections were mediated by 4(th)-generation dendrimers, cationic liposomes and protamine sulfate. Using fluorescence microscopy, we confirmed successful transfections of the nanodiamonds into HeLa cells. Nanodiamond fluorescence could be easily differentiated from cellular autofluorescence. Furthermore, nanodiamonds could be targeted selectively to intracellular structures. Therefore, nanodiamonds are a promising tool for intracellular assays. |
| Databáze: | OpenAIRE |
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