Canine sporotrichosis: polyphasic taxonomy and antifungal susceptibility profiles of Sporothrix species in an endemic area in Brazil
| Autor: | Rodrigo Almeida-Paes, Sandro Antonio Pereira, Isabella Dib Ferreira Gremião, Rosely Maria Zancopé-Oliveira, Paula Gonçalves Viana, Manoel Marques Evangelista Oliveira, Ana Caroline de Sá Machado, Jéssica Sepulveda Boechat |
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| Rok vydání: | 2019 |
| Předmět: |
Posaconazole
Antifungal Agents Itraconazole Microbial Sensitivity Tests Biology Microbiology 03 medical and health sciences Dogs Amphotericin B Media Technology medicine Sporothrix schenckii Animals Dog Diseases skin and connective tissue diseases Terbinafine Phylogeny 030304 developmental biology 0303 health sciences Sporotrichosis 030306 microbiology Sporothrix Broth microdilution Veterinary Microbiology - Research Paper medicine.disease biology.organism_classification bacterial infections and mycoses Ketoconazole Brazil medicine.drug |
| Zdroj: | Braz J Microbiol |
| ISSN: | 1678-4405 |
| Popis: | Sporotrichosis, a mycosis caused by pathogenic species of the genus Sporothrix, affects diverse species of mammals. Until 2007, Sporothrix schenckii was considered the unique etiologic agent of sporotrichosis. Canine sporotrichosis is a poorly reported disease, and the majority of cases are from Rio de Janeiro, Brazil. There are scarce studies on the characterization of canine isolates of Sporothrix schenckii complex, as well as few antifungal susceptibility data available. The aim of this study was to characterize the clinical isolates of Sporothrix from dogs from Brazil at species level and evaluate their antifungal susceptibility profile. Polyphasic taxonomy was used to characterization at species level (morphological, phenotypical characteristics, and molecular identification). Antifungal susceptibility profiles (amphotericin B, itraconazole, ketoconazole, posaconazole, and terbinafine) were determined using the Clinical and Laboratory Standards Institute broth microdilution method (M38-A2). According to phenotypic identification and molecular analysis, 46 isolates included in this study were identified as S. brasiliensis and one as S. schenckii. Amphotericin B presented the highest minimum inhibitory concentration values, and the other drugs showed effective in vitro antifungal activity. This is the first report of S. schenckii in dogs from Brazil, since S. brasiliensis is the only species that has been described in canine isolates from Rio de Janeiro to date. Nevertheless, no differences were observed in the antifungal susceptibility profiles between the S. brasiliensis and S. schenckii isolates, and it is important to continuously study new canine clinical isolates from Rio de Janeiro, Brazil. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s42770-020-00328-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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