Evaluation of cytomorphometric changes in tobacco users and diagnosed oral squamous cell carcinoma individuals
| Autor: | Vijay Srinivas Guduru, Anuradha Ananthaneni, Urmila Udayashankar, Puneeth Horatti Kuberappa, Sabitha Devi Ramisetty, Srilekha Namala |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
Pathology Histology Nuclear area Papanicolaou stain Stain Gastroenterology nuclear area (NA) oral squamous cell carcinoma (OSCC) Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Tobacco users Internal medicine medicine Basal cell lcsh:QH573-671 Image analysis Oral mucosa exfoliative cytology lcsh:Cytology business.industry 030206 dentistry nuclear-to-cellular (N:C) ratio Cellular area (CA) medicine.anatomical_structure 030220 oncology & carcinogenesis Original Article Analysis of variance business |
| Zdroj: | Journal of Cytology / Indian Academy of Cytologists Journal of Cytology, Vol 33, Iss 3, Pp 125-129 (2016) |
| ISSN: | 0970-9371 |
| DOI: | 10.4103/0970-9371.188047 |
| Popis: | Aims: To determine the cellular and nuclear area of keratinocytes in smears obtained from the oral mucosa of tobacco users, those with oral squamous cell carcinoma (OSCC), and from normal healthy persons and resolve if any significant difference exists in these three groups. Materials and Methods: The study group comprised 100 subjects 20 controls, (40 OSCC patients-20 from lesional sites and 20 from nonlesional sites, 20 tobacco smokers and 20 tobacco chewers) in the age group of 25-75 years. Oral mucosal smears obtained by using a cytobrush were stained with Papanicolaou (PAP) stain and using 20X objective in trinocular Olympus model BX53 with Jenoptik scientific grade-dedicated microphotographic camera images were taken. With ProgRes version 8.0 image analysis software, 20 cells with defined borders were evaluated from each slide. Finally, one-way analysis of variance (ANOVA) was used to compare the above parameters in the studied groups. Statistical Analysis Used: Minitab and Excel software were used to analyze the data. One-way ANOVA was used to compare the above parameters in the studied groups. Results: The mean value of the cell area for groups I, II, III, IV, and V were 2838 ± 275.2, 2762.1 ± 511.4, 2861.9 ± 512.9, 2643.8 ± 333.3, and 3064.3 ± 362.7, respectively, the nuclear area (NA) was 83.88 ± 9.86, 106.19 ± 13.45, 95.11 ± 14.24, 85.55 ± 21.11, and 80.83 ± 13.45, respectively, and nuclear-to-cellular (N:C) ratio was 0.0297, 0.03924, 0.0337, 0.03257, and 0.02678, respectively. Conclusions: Thus, our study elucidates that cytomorphology gauges the effect of tobacco on the oral mucosa and possibly establishes a link between premalignant and malignant transformations even before a lesion is visibly noted. |
| Databáze: | OpenAIRE |
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